Whole-mount in situ TUNEL method revealed ectopic pattern of apoptosis in cadmium treated naupliar larvae of barnacle (Balanus amphitrite Darwin)

Shuk Han CHENG, Kwok Wing CHAN, Po Kwok CHAN, Chun Hung SO, Paul K. S. LAM, Shiu Sun Rudolf WU

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effect of cadmium on stage II naupliar larvae of barnacle (Balanus amphitrite Darwin) was investigated. Barnacle larvae were exposed to 0-15 μM CdCl2 for 24 h. Apoptotic cells were stained by the terminal deoxyribonucleotidyl transferase mediated dUTP nick end labelling (TUNEL) method. Incidence of apoptosis, as measured by numbers of animals with ectopic pattern of apoptosis as well as numbers of apoptotic cells per animal, was assessed using confocal microscopy. An increase in incidence of apoptosis was observed in the experimental animals with an increase in cadmium concentration. Mortality increased, and motility decreased, when barnacle larvae were exposed to an increasing concentration of cadmium. The relationship between the occurrence of apoptosis and swimming behaviour was investigated in larvae exposed to 10 μM CdCl2. Significant increases in apoptosis were detected in the non-motile and dead nauplii. This study suggested that whole-mount in situ TUNEL method may be used to study increased occurrence of apoptotic cells in crustacean larvae. Copyright © 2004 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)1387-1394
JournalChemosphere
Volume55
Issue number10
DOIs
Publication statusPublished - Jun 2004

Fingerprint

Thoracica
apoptosis
Cell death
Transferases
Cadmium
Labeling
Larva
cadmium
Apoptosis
larva
Cadmium Chloride
Animals
Cells
animal
swimming behavior
DNA Nucleotidylexotransferase
Confocal microscopy
Incidence
motility
Confocal Microscopy

Citation

Cheng, S. H., Chan, K. W., Chan, P. K., So, C. H., Lam, P. K. S., & Wu, R. S. S. (2004). Whole-mount in situ TUNEL method revealed ectopic pattern of apoptosis in cadmium treated naupliar larvae of barnacle (Balanus amphitrite Darwin). Chemosphere, 55(10), 1387-1394. doi: 10.1016/j.chemosphere.2004.01.011

Keywords

  • Barnacle larvae
  • Cadmium
  • Apoptosis
  • Swimming behaviour
  • TUNEL