The use of cytokine array to examine cytokine profiles of two human cell lines exposed to indoor dust

Yuan KANG, Kwai Chung CHEUNG, Ming Hung WONG

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2 Citations (Scopus)

Abstract

Human cytokine array was used to investigate the cytokine profile of U937 and KERTr after exposure to indoor dust or dust extracts. The release of MCP-1 was increased while release of IL-8 and IL-1β on U937 were decreased after exposure to indoor dust. The releases of RANTES, IL-8 and VEGF from KERTr after exposure to dust extract were increased. The results of IL-8 ELISA assay were consistent with the cytokine array. Real-time RT-PCR was performed to analyze relative changes in gene expression. The MCP-1 mRNA levels were increased after U937 exposure to 18 indoor dust samples, whereas, IL-8 and IL-1β mRNA level showed both up-regulation and down-regulation. The dose-related increase and decrease response was observed on MCP-1 and IL-8, respectively. Most indoor dust extracts increased RANTES, IL-8 and VEGF mRNA levels on KERTr. The dose-dependent response was observed on RANTES and IL-8. A significant correlation (r= 0.48, p< 0.05) was obtained between the total PAHs concentration in dust extracts and the induction of RANTES mRNA. Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)301-307
JournalToxicology Letters
Volume199
Issue number3
DOIs
Publication statusPublished - 15 Dec 2010

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Dust
Interleukin-8
Cells
Chemokine CCL5
Cytokines
Cell Line
Messenger RNA
Interleukin-1
Vascular Endothelial Growth Factor A
Polycyclic aromatic hydrocarbons
Gene expression
Real-Time Polymerase Chain Reaction
Assays
Up-Regulation
Down-Regulation
Enzyme-Linked Immunosorbent Assay
Gene Expression

Citation

Kang, Y., Cheung, K. C., & Wong, M. H. (2010). The use of cytokine array to examine cytokine profiles of two human cell lines exposed to indoor dust. Toxicology Letters, 199(3), 301-307. doi: 10.1016/j.toxlet.2010.09.012

Keywords

  • Human cytokine array
  • U937
  • KERTr