Disassembly is an essential process in supramolecular chemistry, and its exploitation has resulted in the development of four different displacement assays: (a) indicator-displacement assay (IDA), (b) indicator/catalyst-displacement assay (ICDA), (c) catalytic/indicator-displacement assay (CIDA), and (d) catalyst-displacement assay (CDA). These consist of bimetallic donor–acceptor ensembles (BmDAEs), which incorporate various metallic signal transducers, receptors, inhibitors, catalysts, and pre-catalysts. Applications thereof include pollutant degradation, signal amplification, chemosensing detection, and latent catalysis. This review discusses the use of bimetallic supramolecular systems for the development of catalysts, chemosensors, and chemodosimeters. Copyright © 2020 Royal Society of Chemistry.