Abstract
Different concentrations (0·1, 1·6, 2·5, 4·0, 6·3 and 10% w/v) ofaqueous extracts of four waste materials—chicken manure, pig manure, activated sludge and digested sludge—were used as culture media for growing Chlorella pyrenoidosa. Bristol's medium and distilled water were also used for comparison.
Extracts of digested sludge gave the highest growth rate, followed by activated sludge, pig manure, Bristol's medium, distilled water and chicken manure when the concentrations of the waste extracts exceeded 4·0%.
In a second experiment, different concentrations (10⁻⁵, 10⁻⁴, 10⁻³, 10⁻² and 10⁻¹m) of EDTA were added to different waste extracts—chicken manure 2·5%, pig manure 4·0%, activated sludge 6·3%, and digested sludge 10%—prior to algal culture. These concentrations of the various wastes were chosen as they seemed to inhibit algal growth according of the results of the first experiment. Additions of 10⁻⁴ and 10⁻³m EDTA greatly increased algal growth in activated sludge which contained a rather high level of heavy metals (Zn, Cu and Fe).
In a third experiment, two sets of various waste extracts were prepared. One set had been stored for 30 days while the other was kept for 182 days at 20°C before use as culture medium for C. pyrenoidosa. The growth of C. pyrenoidosa was negatively correlated with the content of ammonia-nitrogen in the manure extracts, especially in the case of chicken manure. The 182-day-old chicken manure extract produced a high cell number after the level of ammonia-nitrogen had been diminished. Copyright © 1984 Elsevier Applied Science Publishers Ltd, England.
Extracts of digested sludge gave the highest growth rate, followed by activated sludge, pig manure, Bristol's medium, distilled water and chicken manure when the concentrations of the waste extracts exceeded 4·0%.
In a second experiment, different concentrations (10⁻⁵, 10⁻⁴, 10⁻³, 10⁻² and 10⁻¹m) of EDTA were added to different waste extracts—chicken manure 2·5%, pig manure 4·0%, activated sludge 6·3%, and digested sludge 10%—prior to algal culture. These concentrations of the various wastes were chosen as they seemed to inhibit algal growth according of the results of the first experiment. Additions of 10⁻⁴ and 10⁻³m EDTA greatly increased algal growth in activated sludge which contained a rather high level of heavy metals (Zn, Cu and Fe).
In a third experiment, two sets of various waste extracts were prepared. One set had been stored for 30 days while the other was kept for 182 days at 20°C before use as culture medium for C. pyrenoidosa. The growth of C. pyrenoidosa was negatively correlated with the content of ammonia-nitrogen in the manure extracts, especially in the case of chicken manure. The 182-day-old chicken manure extract produced a high cell number after the level of ammonia-nitrogen had been diminished. Copyright © 1984 Elsevier Applied Science Publishers Ltd, England.
| Original language | English |
|---|---|
| Pages (from-to) | 55-71 |
| Journal | Environmental Pollution Series A: Ecological and Biological |
| Volume | 34 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1984 |