Successive transformation of benzo[a]pyrene by laccase of Trametes versicolor and pyrene-degrading Mycobacterium strains

Jun ZENG, Xiangui LIN, Jing ZHANG, Hong ZHU, Hong CHEN, Ming Hung WONG

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11 Citations (Scopus)

Abstract

We previously hypothesized that polycyclic aromatic hydrocarbon (PAH)-degrading bacteria that produce laccase may enhance the degree of benzo[a]pyrene mineralization. However, whether the metabolites of benzo[a]pyrene oxidized by laccase can be further transformed by PAH degraders remains unknown. In this study, pyrene-degrading mycobacteria with diverse degradation properties were isolated and employed for investigating the subsequent transformation on the metabolites of benzo[a]pyrene oxidized by fungal laccase of Trametes versicolor. The results confirm the successive transformation of benzo[a]pyrene metabolites, 6-benzo[a]pyrenyl acetate, and quinones by Mycobacterium strains, and report the discovery of the involvement of a O-methylation mediated pathway in the process. In detail, the vast majority of metabolite 6-benzo[a]pyrenyl acetate was transformed into benzo[a]pyrene quinones or methoxybenzo[a]pyrene, via two distinct steps that were controlled by the catechol-O-methyltransferase mediated O-methylation, while quinones were reduced to dihydroxybenzo[a]pyrene and further transformed into dimethoxy derivatives. Copyright © 2012 Springer-Verlag.

Original languageEnglish
Pages (from-to)3183-3194
JournalApplied Microbiology and Biotechnology
Volume97
Issue number7
Early online date11 May 2012
DOIs
Publication statusPublished - Apr 2013

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Trametes
Laccase
Benzo(a)pyrene
Mycobacterium
Quinones
Polycyclic Aromatic Hydrocarbons
Methylation
Acetates
Catechol O-Methyltransferase
pyrene
Bacteria

Citation

Zeng, J., Lin, X., Zhang, J., Zhu, H., Chen, H., & Wong, M. H. (2013). Successive transformation of benzo[a]pyrene by laccase of Trametes versicolor and pyrene-degrading Mycobacterium strains. Applied Microbiology and Biotechnology, 97(7), 3183-3194. doi: 10.1007/s00253-012-4120-2

Keywords

  • Benzo[a]pyrene
  • Fungal laccase
  • Mycobacterium
  • Successive transformation