Statistical study on the effects of environmental factors on the growth and microcystins production of bloom-forming cyanobacterium: Microcystis aeruginosa

Y. JIANG, B. JI, R.N.S. WONG, Ming Hung WONG

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Abstract

In this paper, a systematic investigation on the effects of environmental factors on the growth and microcystins production of Microcystis aeruginosa using statistically based experimental design is presented. The effects of various chemical and physical factors on cell growth and microcystins production were firstly studied in two levels Plackett-Burman design. NaNO₃, K₂HPO₄, iron, light intensity and temperature were screened out to have significant effects (with confidence level above 90%) on cell growth whereas only NaNO₃, iron and light intensity had significant effects on microcystin production. The interactions between the screened variables were then evaluated using central composite design. The analysis of the experimental responses revealed that nitrate and phosphate as well as phosphate and temperature had significant interactive effects on cell growth, respectively, whereas iron and light had significant interactive effect on microcystins production. The critical border values of the variables that may stimulate the rapid growth of Microcystis and microcystin production, when they were simultaneous present in the water, were represented in the second-order polynomial models and determined to be NaNO₃ >1.27 mM, K₂HPO₄ >0.1 mM, iron >0.01 mM, light intensity >26 μmol m⁻² s⁻¹ as well as temperature >18.8 °C, respectively. Copyright © 2007 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)127-136
JournalHarmful Algae
Volume7
Issue number2
DOIs
Publication statusPublished - Feb 2008

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microcystins
Microcystis aeruginosa
Cyanobacteria
cyanobacterium
algal bloom
environmental factor
environmental factors
iron
light intensity
cell growth
phosphates
temperature
phosphate
Microcystis
experimental design
nitrates
effect
nitrate
water

Citation

Jiang, Y., Ji, B., Wong, R. N. S., & Wong, M. H. (2008). Statistical study on the effects of environmental factors on the growth and microcystins production of bloom-forming cyanobacterium: Microcystis aeruginosa. Harmful Algae, 7(2), 127-136. doi: 10.1016/j.hal.2007.05.012

Keywords

  • Microcystis aeruginosa
  • Environmental factors
  • Microcystins
  • Factorial design