Sludge-grown algae for culturing aquatic organisms: Part I. Algal growth in sludge extracts

K. M. HUNG, S. T. CHIU, Ming Hung WONG

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This project is aimed at studying the feasibility of using sewage sludge to prepare culture media for microalgae (Chlorella-HKBU) and the use of the sludge grown algae as a feed for some aquatic organisms Part I of the project included results on preparing sludge extracts and their use on algal culture. By comparing two culturing techniques, 'aeration' and 'shaking,' it was noted that both lag and log phases were shortened in the aeration system. A subsequent experiment noted that algal growth subject to aeration rates of 1.0 and 1.5 liters/min had similar lag and log phases. In addition, both aeration rates had a significantly higher (P < 005) final cell density than that of 05 liters/min. A detailed study on the variation of growth conditions on the algal growth was done. The results indicated that pH values of all the cultures declined below 5 at day 12. The removal rates of ammonia N ranged from 62% to 70%. The sludge-grown algae contained a rather substantial amount of heavy metals (μg/g): Zn 289-581, Cu 443-682, Ni 310-963, Mn 96-126, Cr 25-118, and Fe 438-653. This implied that the rather high levels of heavy metals may impose adverse effects on higher trophic organisms. Copyright © 1996 Kluwer Academic Publishers.

Original languageEnglish
Pages (from-to)361-374
JournalEnvironmental Management
Issue number3
Publication statusPublished - May 1996



Hung, K. M., Chiu, S. T., & Wong, M. H. (1996). Sludge-grown algae for culturing aquatic organisms: Part I. Algal growth in sludge extracts. Environmental Management, 20(3), 361-374. doi: 10.1007/BF01203844


  • Algal growth
  • Chlorella
  • Heavy metals
  • Sludge extracts