Simultaneous determination of oxytetra-cycline, doxycycline, tetracycline and chlortetracycline in tetracycline antibiotics by high-performance liquid chromatog-raphy with fluorescence detection

Hai-Tao LU, Yue JIANG, Hua-Bin LI, Feng CHEN, Ming Hung WONG

Research output: Contribution to journalArticles

23 Citations (Scopus)

Abstract

A simple, rapid and sensitive high-performance liquid chromatographic method with fluorescence detection for the simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline was developed, and successfully applied to the analysis of commercial tetracycline antibiotics. The separation was performed on a reverse-phase C₁₈ column with a gradient elution composed of methanol and sodium acetate buffer (containing disodium ethylenediaminetetraacetate and calcium chloride, pH 8.10) as the mobile phase, and fluorescence detection at 532 nm (excitation at 380 nm). The detection limits for oxytetracycline, doxycycline, tetracycline and chlortetracycline were 0.1, 0.5, 0.3 and 0.4 μg L⁻¹, respectively. Data with respect to precision and accuracy were reported and discussed. Copyright © 2004 Friedr. Vieweg&Sohn/GWV Fachverlage GmbH.
Original languageEnglish
Pages (from-to)259-264
JournalChromatographia
Volume60
Issue number5-6
DOIs
Publication statusPublished - Sep 2004

Citation

Lu, H.-T., Jiang, Y., Li, H.-B., Chen, F., & Wong, M.-H. (2004). Simultaneous determination of oxytetra-cycline, doxycycline, tetracycline and chlortetracycline in tetracycline antibiotics by high-performance liquid chromatog-raphy with fluorescence detection. Chromatographia, 60(5-6), 259-264. doi: 10.1365/s10337-004-0356-z

Keywords

  • Column liquid chromatography
  • Fluorescence detection
  • Tetracycline antibiotics
  • Pharmaceuticals

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