Silver nanoparticles disrupt regulation of steroidogenesis in fish ovarian cells

Natalie DEGGER, Anna C. K. TSE, Shiu Sun Rudolf WU

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13 Citations (Scopus)

Abstract

Despite the influx of silver nanoparticles (nAg) into the marine environment, their effects on fish reproduction remain completely unexplored. Using ovarian primary cells from marine medaka (Oryzias melastigma), in vitro studies were carried out to evaluate the effects of two differently coated nAg particles (Oleic Acid, (OA) nAg and Polyvinylpyrrolidone, (PVP) nAg) on fish ovarian tissues, using AgNO3 as a positive control. Cytotoxicity was evaluated by MTT assay and expression of key genes regulating steroidogenesis (StAR, CYP 19a, CYP 11a, 3βHSD and 20βHSD) were determined by Q-RT-PCR. EC50 values for PVP nAg, OA nAg and AgNO3 were 7.25μgL-1, 924.4μgL-1, and 42.0μgL-1 respectively, showing that toxicity of silver was greatly enhanced in the PVP coated nano-form. Down regulation of CYP 19a was observed in both nAg and AgNO3 treatments, while down regulation of 3βHSD was only found in the OA nAg and AgNO3 treatments. For the first time, our results demonstrated that nAg can affect specific genes regulating steroidogenesis, implicating nAg as a potential endocrine disruptor. Copyright © 2015 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)143-151
JournalAquatic Toxicology
Volume169
Early online date11 Nov 2015
DOIs
Publication statusPublished - Dec 2015

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nanosilver
Povidone
steroidogenesis
Oleic Acid
Silver
Nanoparticles
Oryzias
oleic acid
silver
Fishes
acid
Down-Regulation
fish
Endocrine Disruptors
endocrine-disrupting chemicals
endocrine disruptor
gene
cells
marine environment
in vitro studies

Citation

Degger, N., Tse, A. C. K., & Wu, R. S. S. (2015). Silver nanoparticles disrupt regulation of steroidogenesis in fish ovarian cells. Aquatic Toxicology, 169, 143-151. doi: 10.1016/j.aquatox.2015.10.015

Keywords

  • Silver nanoparticles
  • Steroidogenesis
  • Marine medaka
  • Endocrine disruption
  • Primary cell culture