Role of electron donor in CO₂ fixation of chemoautotrophic bacteria and its preconditions: Verification in Alcaligenes hydrogenophilus

Saiwei ZHANG, Ya-Nan WANG, Lei WANG, Yiu Fai TSANG, Huan LI, Xiaohua FU

Research output: Contribution to journalArticle

Abstract

Alcaligenes hydrogenophilus was used to verify the role of the electron donor and acceptor in apparent CO₂ fixation of chemoautotrophic bacteria. The response mechanisms underlying the apparent CO₂ fixation characteristics with different concentrations of electron donor and acceptor were elucidated by analyzing the transcription characteristics of the cbbL gene, cytoskeleton synthesis efficiency and extracellular free organic carbon concentration. The results showed that the apparent CO₂ fixation efficiency of A. hydrogenophilus was significantly influenced by the electron donor (H₂), and the degree of electron donor oxidization was responsible for the variation in apparent CO₂ fixation efficiency. Furthermore, transcription efficiency of the cbbL gene at low electron donor concentration was lower than that at high electron donor concentration, but excessive electron donor concentrations did not further increase cbbL gene transcription efficiency significantly. High oxygen concentration was not advantageous to cbbL gene transcription efficiency in A. hydrogenophilus, but could improve cell growth rate (protein synthesis rate) and apparent CO₂ fixation efficiency, implying that cytoskeleton synthesis efficiency is another important factor determining apparent CO₂ fixation efficiency and its contribution maybe greater than that of cbbL transcription. The results also indicated that high apparent CO₂ fixation efficiency required the matching of electron donor and acceptor. Copyright © 2018 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)37-43
JournalEnzyme and Microbial Technology
Volume118
Early online date28 Jun 2018
DOIs
Publication statusPublished - Nov 2018

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Citation

Zhang, S., Wang, Y.-N., Wang, L., Tsang, Y. F., Li, H., & Fu, X. (2018). Role of electron donor in CO₂ fixation of chemoautotrophic bacteria and its preconditions: Verification in Alcaligenes hydrogenophilus. Enzyme and Microbial Technology, 118, 37-43. doi: 10.1016/j.enzmictec.2018.06.010

Keywords

  • Alcaligenes hydrogenophilus
  • H₂
  • Electron donor
  • Apparent CO₂ fixation efficiency
  • cbbL gene