Responses of the medaka HPG axis PCR array and reproduction to prochloraz and ketoconazole

Xiaowei ZHANG, Markus HECKER, Paul D. JONES, John NEWSTED, Doris AU, Richard KONG, Shiu Sun Rudolf WU, John P. GIESY

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Effects of two model imidazole-type fungicides, prochloraz (PCZ) and ketoconazole (KTC), on the hypothalamic-pituitary-gonadal (HPG) axis of the Japanese medaka (Oryzias latipe) were examined by use of real time PCR (RT-PCR) array, Fourteen-week-old Japanese medaka were exposed for seven days to concentrations of PCZ or KTC from 3.0 to 300 μg/L Exposure to KTC or PCZ caused significant reduction of fecundity of Japanese medaka and down-regulated expression of estrogen receptor (ER)-α and egg precursors in livers of males and females. However, PCZ was more potent than KTC both in modulating transcription and causing lesser fecundity. Exposure to nominal 30 μg PCZ/L resulted in 50% less fecundity and significant down-regulation of vitellogenin // expression, but KTC did not cause such effects at this concentration. Exposure to PCZ caused a compensatory upregulation in cytochrome P450 c17αhydroxylase, 17,20-lyase (CYP17) and aromatase (CYP19) expression in the ovary, while KTC did not. Furthermore, the ecologically relevant end point, fecundity was log-log related to mRNA level of six genes in livers of females. Copyright © 2008 American Chemical Society.

Original languageEnglish
Pages (from-to)6762-6769
JournalEnvironmental Science and Technology
Volume42
Issue number17
DOIs
Publication statusPublished - 2008

Citation

Zhang, X., Hecker, M., Jones, P. D., Newsted, J., Au, D., Kong, R., . . . Giesy, J. P. (2008). Responses of the medaka HPG axis PCR array and reproduction to prochloraz and ketoconazole. Environmental Science and Technology, 42(17), 6762-6769. doi: 10.1021/es800591t

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