Quantitative analysis of nicotinic acid, nicotinamide and 3-cyanopyridine in industrial effluent by high performance liquid chromatography

Po Keung Eric TSANG, Lixuan ZENG

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A rapid and reliable high performance liquid chromatographic (HPLC) method for the simultaneous determination of heterocyclic compounds, namely nicotinic acid, nicotinamide, and 3-cyanopyridine, in industrial effluent is described. A Φ4.6 mm × 150 mm, 5 μm C-18 reversed phase stationery phase, and a methanol-acetonitrile-water tertiary mobile phase (20:20:60 v/v) were used for separation. The detection wavelength of a diode array (DAD) was set at 216 nm with a bandwidth of 16 nm. Phenol was used as an internal standard. The regression equations revealed a linear relationship between the concentration of the analytes injected and the peak area detected by DAD. The limits of detection (S/N = 3) ranged from 0.70 to 1.18 mg L−1, the recoveries ranged from 87% to 102% and the precision expressed as % RSD intra-day and inter-day varied from 0.9 to 3.9 and 1.2 to 5.6, respectively. This method is rapid, sensitive and suitable for the monitoring of nicotinic acid, nicotinamide, and 3-cyanopyridine in effluent of related pharmaceutical manufacturing plants. Copyright © 2007 Higher Education Press and Springer-Verlag.
Original languageEnglish
Pages (from-to)270-273
JournalFrontiers of Chemistry in China
Volume2
Issue number3
DOIs
Publication statusPublished - Jul 2007

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Niacinamide
Niacin
High performance liquid chromatography
Effluents
Heterocyclic Compounds
Phenol
Chemical analysis
Methanol
Diodes
Bandwidth
Recovery
Wavelength
Water
Monitoring
Liquids
Pharmaceutical Preparations
3-cyanopyridine
acetonitrile

Citation

Tsang, P. K., & Zeng, L. (2007). Quantitative analysis of nicotinic acid, nicotinamide and 3-cyanopyridine in industrial effluent by high performance liquid chromatography. Frontiers of Chemistry in China, 2(3), 270-273.

Keywords

  • Effluent
  • Heterocyclic compounds
  • High performance liquid chromatographic method