Prevalence and diversity of norovirus genogroups I and II in Hong Kong marine waters and detection by real-time PCR

Ning YANG, Huizhou QI, Minnie Man Lai WONG, Shiu Sun Rudolf WU, Richard Yuen Chong KONG

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Marine waters from six sites around Hong Kong with varying levels of sewage pollution were examined for noroviruses (NoVs) by PCR cloning and sequencing of a highly-variable N-terminal region of the VP1 capsid gene, at the ORF1-ORF2 junction of NoV. Phylogenetic analysis of genogroups GI- and GII-specific PCR clones obtained from different marine sites indicated that human NoV GI.1 and GII.4 strains are the most prevalent genotypes circulating in Hong Kong waters. GI- and GII-specific TaqMan-based real-time PCR assays targeting the ORF1-ORF2 junction of NoVs were used to quantify NoV particles in marine water samples in parallel with total Escherichia coli counts which were enumerated on TBX medium. No correlation of any significance between NoV and E. coli counts was observed which highlighted the inadequacy in using E. coli as a fecal indicator to predict the level of NoVs in marine waters to protect public health. Copyright © 2011 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)164-168
JournalMarine Pollution Bulletin
Volume64
Issue number1
Early online date25 Nov 2011
DOIs
Publication statusPublished - Jan 2012

Fingerprint

Norovirus
quantitative polymerase chain reaction
Escherichia coli
China
Water
capsid
sewage
water
molecular cloning
Cloning
public health
Public health
Sewage
pollution
clones
targeting
clone
Assays
Pollution
genotype

Citation

Yang, N., Qi, H., Wong, M. M. L., Wu, R. S. S., & Kong, R. Y. C. (2012). Prevalence and diversity of norovirus genogroups I and II in Hong Kong marine waters and detection by real-time PCR. Marine Pollution Bulletin, 64(1), 164-168. doi: 10.1016/j.marpolbul.2011.10.037

Keywords

  • Noroviruses
  • NoV GI.1
  • NoV GII.4
  • TaqMan real-time PCR
  • Hong Kong marine waters
  • Fecal contamination