Potential cytotoxicity of water-soluble fraction of dust and particulate matters and relation to metal(loid)s based on three human cell lines

Minjuan HUANG, Yuan KANG, Wei WANG, Chuen Yu CHAN, Xuemai WANG, Ming Hung WONG

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11 Citations (Scopus)

Abstract

Hepatocellular liver carcinoma (HepG2), human skin derived keratinocyte (KERTr,) and lung epithelial carcinoma (A549) were employed in MTT assay to evaluate the cytotoxicity of water-soluble fraction of road dust, air-conditioning (AC) filter dust and PM2.5 via ingestion, dermal contact and inhalation. Their effects on cell growth were dependent on exposure time and concentration. The LC20s of PM2.5 for A549 cell were approximately one order of magnitude lower than those of road dust and AC filter dust for KERTr cell and HepG2 cell. The LC20s of aqueous extracts were negatively correlated to the water-soluble metal(loid)s contained in dust coarse particles (KERTr: p = 0.004; HepG2: p < 0.001). However, no significant correlation between soluble metal(loid)s and LC20s of PM2.5 was observed for A549 cell (p > 0.05). Other water-soluble components in dust and PM might cause the cell hazards synergistically or additively with metal(loid)s. Copyright © 2015 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)61-66
JournalChemosphere
Volume135
Early online date22 Apr 2015
DOIs
Publication statusPublished - Sep 2015

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particulate matter
dust
metal
air conditioning
water
road
filter
cytotoxicity
skin
hazard
assay

Bibliographical note

Huang, M., Kang, Y., Wang, W., Chan, C. Y., Wang, X., & Wong, M. H. (2015). Potential cytotoxicity of water-soluble fraction of dust and particulate matters and relation to metal(loid)s based on three human cell lines. Chemosphere, 135, 61-66. doi: 10.1016/j.chemosphere.2015.04.004.

Keywords

  • Cytotoxicity
  • Water-soluble metal(loid)s
  • Dust and PM
  • HepG2
  • KERTr
  • A549