Polybrominated diphenyl ethers do not affect metamorphosis but alter the proteome of the invasive slipper limpet Crepidula onyx

Joy MUKHERJEE, Beverly H. K. PO, Jill M. Y. CHIU, Shiu Sun Rudolf WU, Pei-Yuan QIAN, Vengatesen THIYAGARAJAN

Research output: Contribution to journalArticles

3 Citations (Scopus)

Abstract

Man-made polybrominated diphenyl ethers (PBDEs) used as flame retardants in various consumer products may be harmful to marine organisms. Larvae of some marine invertebrates, especially invasive species, can develop resistance to PBDEs through altered protein expression patterns or proteome plasticity. This is the first report of a proteomics approach to study BDE-47 induced molecular changes in the invasive limpet Crepidula onyx. Larvae of C. onyx were cultured for 5days (hatching to metamorphosis) in the presence of BDE-47 (1μgL-1). Using a 2-DE proteomics approach with triple quadrupole and high-resolution TOF-MS, we showed that BDE-47 altered the proteome structure but not the growth or metamorphosis of C. onyx larvae. We found eight significant differentially expressed proteins in response to BDE-47, deemed the protein expression signature, consisting of cytoskeletal, stress tolerance, metabolism and energy production related proteins. Our data suggest C. onyx larvae have adequate proteome plasticity to tolerate BDE-47 toxicity. Copyright © 2013 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)273-281
JournalMarine Pollution Bulletin
Volume73
Issue number1
Early online date03 Jun 2013
DOIs
Publication statusPublished - 15 Aug 2013

Citation

Mukherjee, J., Po, B. H. K., Chiu, J. M. Y., Wu, R. S. S., Qian, P.-Y., & Thiyagarajan, V. (2013). Polybrominated diphenyl ethers do not affect metamorphosis but alter the proteome of the invasive slipper limpet Crepidula onyx. Marine Pollution Bulletin, 73(1), 273-281. doi: 10.1016/j.marpolbul.2013.05.009

Keywords

  • Crepidula onyx
  • Polybrominated diphenyl ethers (PBDEs)
  • Larval metamorphosis
  • Environmental proteomics
  • Protein expression signatures

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