Photoresponsive molecularly imprinted hydrogel casting membrane for the determination of trace tetracycline in milk

Qiang WANG, Zhen LV, Qian TANG, Cheng-Bin GONG, Michael Hon Wah LAM, Xue-bing MA, Cheuk Fai Stephen CHOW

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This study aimed to develop a photoresponsive molecularly imprinted hydrogel (MIH) casting membrane for the determination of trace tetracycline (TC) in milk. This MIH casting membrane combined the specificity of MIHs, the photoresponsive properties of azobenzene, and the portable properties of a membrane. Photoresponsive TC-imprinted MIHs were initially fabricated and then cast on sodium dodecyl sulfonate polyacrylamide gel. After TC removal, a photoresponsive MIH casting membrane was obtained. The photoresponsive properties of the MIH casting membrane were robust, and no obvious photodegradation was observed after 20 cycles. The MIH casting membrane displayed specific affinity to TC upon alternate irradiation at 365 and 440 nm; it could quantitatively uptake and release TC. The TC concentration (0.0–2.0 × 10⁻⁴ mol l⁻¹) in aqueous solution displayed a linear relationship with the photoisomerization rate constant of azobenzene within the MIH casting membrane. As such, a quick detection method for trace TC in aqueous foodstuff samples was established. The recovery of this method for TC in milk was investigated with a simple pretreatment of milk, and a high recovery of 100.54–106.35% was obtained. Therefore, the fabricated membrane can be used as a portable molecular sensor that can be easily recycled. Copyright © 2015 John Wiley & Sons, Ltd.
Original languageEnglish
Pages (from-to)123-130
JournalJournal of Molecular Recognition
Volume29
Issue number3
Early online dateMar 2015
DOIs
Publication statusPublished - Mar 2016

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Hydrogel
Tetracycline
Milk
Membranes
Photolysis
Sodium

Bibliographical note

Wang, Q., Lv, Z., Tang, Q., Gong, C.-B., Lam, M. H. W., Ma, X.-B. et al. (2016). Photoresponsive molecularly imprinted hydrogel casting membrane for the determination of trace tetracycline in milk. Journal of Molecular Recognition, 29(3), 123-130.

Keywords

  • Photoresponsive MIHS casting membrane
  • Tetracycline antibiotics
  • 4–[(4–methacryloyloxy)phenylazo] benzenesulfonic acid
  • Molecularly imprinted hydrogels