Overexpression and knockdown of hypoxia-inducible factor 1 disrupt the expression of steroidogenic enzyme genes and early embryonic development in zebrafish

Tianfeng TAN, Richard Man Kit YU, Shiu Sun Rudolf WU, Richard Yuen Chong KONG

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9 Citations (Scopus)

Abstract

Hypoxia is an important environmental stressor leading to endocrine disruption and reproductive impairment in fish. Although the hypoxia-inducible factor 1 (HIF-1) is known to regulate the transcription of various genes mediating oxygen homeostasis, its role in modulating steroidogenesis-related gene expression remains poorly understood. In this study, the regulatory effect of HIF-1 on the expression of 9 steroidogenic enzyme genes was investigated in zebrafish embryos using a "gain-of-function and loss-of-function" approach. Eight of the genes, CYP11a, CYP11b2, 3β-HSD, HMGCR, CYP17a1, 17β-HSD2, CYP19a, and CYP19b, were found to be differentially upregulated at 24 and 48 hpf following zHIF-1α-ΔODD overexpression (a mutant zebrafish HIF-1α protein with proline-414 and proline-557 deleted). Knockdown of zHIF-1α also affected the expression pattern of the steroidogenic enzyme genes. Overexpression of zHIF-1α and hypoxia exposure resulted in downregulated StAR expression but upregulated CYP11a and 3β-HSD expression in zebrafish embryos. Conversely, the expression patterns of these 3 genes were reversed in embryos in which zHIF-1α was knocked down under normoxia, suggesting that these 3 genes are regulated by HIF-1. Overall, the findings from this study indicate that HIF-1–mediated mechanisms are likely involved in the regulation of specific steroidogenic genes. Copyright © 2017 The Author(s).
Original languageEnglish
JournalGene Regulation and Systems Biology
Volume11
Early online dateJun 2017
DOIs
Publication statusPublished - 2017

Citation

Tan, T., Yu, R. M. K., Wu, R. S. S., & Kong, R. Y. C. (2017). Overexpression and knockdown of hypoxia-inducible factor 1 disrupt the expression of steroidogenic enzyme genes and early embryonic development in zebrafish. Gene Regulation and Systems Biology, 11. Retrieved from http://dx.doi.org/10.1177/1177625017713193

Keywords

  • HIF-1
  • Hypoxia
  • Steroidogenic enzyme genes
  • Zebrafish
  • Steroid hormones

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