Molecular staging of marine medaka: A model organism for marine ecotoxicity study

Xueping CHEN, Li LI, Jinping CHENG, Leo Lai CHAN, Da-Zhi WANG, Ke-Jian WANG, Michael E. BAKER, Gary HARDIMAN, Daniel SCHLENK, Shuk Han CHENG

Research output: Contribution to journalArticlespeer-review

42 Citations (Scopus)

Abstract

Oryzias melastigma, also called O. dancena, is becoming a very useful model for estuarine and marine ecotoxicity studies. With O. melastigma being adopted by ILSI Health and Environmental Science Institute (HESI) for embryo toxicity testing, improved knowledge of biomarker based embryonic development becomes especially important for mechanism-based toxicity evaluations. Using whole mount in situ hybridization and immunostaining techniques together with widely used molecular markers, this study describes the molecular development of marine medaka embryos, focusing on the brain, eye, heart, pectoral fin, pancreas, liver, muscle and neuron system. These organs are targets of environmental pollutants that disrupt normal embryonic development in medaka and other fish. Copyright © 2011 Elsevier Ltd.
Original languageEnglish
Pages (from-to)309-317
JournalMarine Pollution Bulletin
Volume63
Issue number5-12
Early online date25 Jun 2011
DOIs
Publication statusPublished - 2011

Citation

Chen, X., Li, L., Cheng, J., Chan, L. L., Wang, D.-Z., Wang, K.-J., . . . Cheng, S. H. (2011). Molecular staging of marine medaka: A model organism for marine ecotoxicity study. Marine Pollution Bulletin, 63(5-12), 309-317. doi: 10.1016/j.marpolbul.2011.03.042

Keywords

  • Medaka
  • Oryzias melastigma
  • Molecular staging
  • In situ hybridization
  • Immunostaining

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