Molecular interaction kinetics and mechanism study of phytohormones and plant protein with fluorescence and synchronous fluorescence techniques

Yuan-Yuan ZHENG, Ning SUN, Miao-Han XU, Yu-Jing LU, Bin QIU, Ming-Jun CHENG, Wing Leung WONG, Cheuk Fai Stephen CHOW

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Abstract

Cytokinins (CTKs) are widely used in agriculture to enhance crop production. To understand the role of CTKs in plant growth is vital, however, molecular mechanisms underlying CTKs action particularly their binding kinetics with proteins are not clear. The study of these interactions can obtain important information on how these signal molecules regulate cellular processes in targeted cells locally or transport to other functional parts of plants. In this study, the interactions between two CTKs and soy protein were investigated. The binding constants determined at 30 °C were Kκᴛ-ѕᴏу = 1.5×10³ M⁻¹ and Kıᴘ-ѕᴏу=0.67×10² M⁻¹ and the binding stoichiometry was nκᴛ-ѕᴏу=0.530 and nıᴘ-ѕᴏу=0.5165 respectively. Moreover, the CTK-protein interaction is found to be a static quenching process via an energy transfer mechanism. The adduct formed through electrostatic interactions under the plant acidity conditions was thus transported to the action parts of the plant to control growth process. Copyright © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Original languageEnglish
Pages (from-to)3993-4000
JournalChemistrySelect
Volume2
Issue number14
DOIs
Publication statusPublished - May 2017

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Cytokinins
Plant Proteins
Plant Growth Regulators
Molecular interactions
Fluorescence
Kinetics
Soybean Proteins
Coulomb interactions
Acidity
Stoichiometry
Energy transfer
Agriculture
Crops
Quenching
Proteins
Molecules

Bibliographical note

Zhang, Y.-Y., Sun, N., Xu, M.-H., Lu, Y.-J., Qiu, B., Cheng, M.-J., et al. (2017). Molecular interaction kinetics and mechanism study of phytohormones and plant protein with fluorescence and synchronous fluorescence techniques. ChemistrySelect, 2(14), 3993-4000.

Keywords

  • Fluorescence spectroscopy
  • Kinetin (KT)
  • N6-isopentenyl adenine (IP)
  • Soy protein
  • Static quenching