Molecular cloning and characterization of a hypoxia-responsive CITED3 cDNA from grass carp

P. K. S. NG, Shiu Sun Rudolf WU, Z. P. ZHANG, H. O. L. MOK, D. J. RANDALL, R. Y. C. KONG

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7 Citations (Scopus)

Abstract

We have isolated a 1586-bp full-length CITED3 cDNA from grass carp which specifies for a cAMP-responsive element-binding protein/p300-interacting transactivator with glutamic acid (E)/aspartic acid (D)-rich C-terminal domain protein. The cDNA, designated as gcCITED3, has an open reading frame of 762 bp and encodes a protein of 253 amino acids with a predicted molecular mass of 28. 3 kDa and pI of 6.4. Pairwise comparison showed that gcCITED3 shares high sequence identity with the CITED3 of zebrafish (94%), chicken (72%) and Xenopus (59%). Northern blot analysis indicated that gcCITED3 is most highly expressed and responsive to hypoxia in the carp kidney. Hypoxic induction was also observed in heart, albeit at a lower level. This is the first report on the isolation of a hypoxia-responsive CITED3 gene from fish. Copyright © 2003 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)163-172
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume136
Issue number2
DOIs
Publication statusPublished - Oct 2003

Citation

Ng, P. K. S., Wu, R. S. S., Zhang, Z. P., Mok, H. O. L., Randall, D. J., & Kong, R. Y. C. (2003). Molecular cloning and characterization of a hypoxia-responsive CITED3 cDNA from grass carp. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 136(2), 163-172. doi: 10.1016/S1096-4959(03)00224-0

Keywords

  • Ctenopharyngodon idellus
  • CITED
  • Grass carp
  • Hypoxia
  • Gene regulation
  • Co-transactivator
  • RACE PCR

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