Molecular cloning and characterization of a hypoxia-responsive CITED3 cDNA from grass carp

P. K. S. NG, Shiu Sun Rudolf WU, Z. P. ZHANG, H. O. L. MOK, D. J. RANDALL, R. Y. C. KONG

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Abstract

We have isolated a 1586-bp full-length CITED3 cDNA from grass carp which specifies for a cAMP-responsive element-binding protein/p300-interacting transactivator with glutamic acid (E)/aspartic acid (D)-rich C-terminal domain protein. The cDNA, designated as gcCITED3, has an open reading frame of 762 bp and encodes a protein of 253 amino acids with a predicted molecular mass of 28. 3 kDa and pI of 6.4. Pairwise comparison showed that gcCITED3 shares high sequence identity with the CITED3 of zebrafish (94%), chicken (72%) and Xenopus (59%). Northern blot analysis indicated that gcCITED3 is most highly expressed and responsive to hypoxia in the carp kidney. Hypoxic induction was also observed in heart, albeit at a lower level. This is the first report on the isolation of a hypoxia-responsive CITED3 gene from fish. Copyright © 2003 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)163-172
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume136
Issue number2
DOIs
Publication statusPublished - Oct 2003

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Carps
Cloning
Molecular Cloning
Complementary DNA
D-Aspartic Acid
Dilatation and Curettage
Trans-Activators
Molecular mass
Zebrafish
Xenopus
Aspartic Acid
Northern Blotting
Fish
Open Reading Frames
Glutamic Acid
Chickens
Carrier Proteins
Fishes
Proteins
Genes

Citation

Ng, P. K. S., Wu, R. S. S., Zhang, Z. P., Mok, H. O. L., Randall, D. J., & Kong, R. Y. C. (2003). Molecular cloning and characterization of a hypoxia-responsive CITED3 cDNA from grass carp. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 136(2), 163-172. doi: 10.1016/S1096-4959(03)00224-0

Keywords

  • Ctenopharyngodon idellus
  • CITED
  • Grass carp
  • Hypoxia
  • Gene regulation
  • Co-transactivator
  • RACE PCR