We have isolated a 1586-bp full-length CITED3 cDNA from grass carp which specifies for a cAMP-responsive element-binding protein/p300-interacting transactivator with glutamic acid (E)/aspartic acid (D)-rich C-terminal domain protein. The cDNA, designated as gcCITED3, has an open reading frame of 762 bp and encodes a protein of 253 amino acids with a predicted molecular mass of 28. 3 kDa and pI of 6.4. Pairwise comparison showed that gcCITED3 shares high sequence identity with the CITED3 of zebrafish (94%), chicken (72%) and Xenopus (59%). Northern blot analysis indicated that gcCITED3 is most highly expressed and responsive to hypoxia in the carp kidney. Hypoxic induction was also observed in heart, albeit at a lower level. This is the first report on the isolation of a hypoxia-responsive CITED3 gene from fish. Copyright © 2003 Elsevier Inc. All rights reserved.
|Journal||Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology|
|Publication status||Published - Oct 2003|
CitationNg, P. K. S., Wu, R. S. S., Zhang, Z. P., Mok, H. O. L., Randall, D. J., & Kong, R. Y. C. (2003). Molecular cloning and characterization of a hypoxia-responsive CITED3 cDNA from grass carp. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 136(2), 163-172. doi: 10.1016/S1096-4959(03)00224-0
- Ctenopharyngodon idellus
- Grass carp
- Gene regulation
- RACE PCR