A multi-channel thermal activity microcalorimeter was used to determine the pyrene-induced toxic effect on two polycyclic aromatic hydrocarbon (PAH)-degrading bacteria Acinetobacter junii (A. junii) and Bacillus subtilis (B. subtilis). Power-time curves were analyzed and calorimetric parameters including growth rate constant (k), half inhibitory concentration (IC ₅₀) and total thermal effect (Q т ) were obtained. A. junii and B. subtilis were completely inhibited when the concentration of pyrene reached 400 and 160 μ g mL⁻¹, respectively. The relationships between the calorimetric parameters and concentration of pyrene were studied. The growth rate of A. junii decreased with the increase in pyrene concentration at 50–200 μ g mL⁻¹. The growth of biomass for A. junii at various concentrations of pyrene was determined. The count of A. junii after 8 day's incubation reached maximum irrespective of the initial pyrene concentrations ranging from 50 to 200 μ g mL⁻¹ and the smallest stimulative action of pyrene was at 200 μ g mL⁻¹. The variations of biomass during the growth of A. junii were consistent with the microcalorimetric data, indicating that microcalorimetry can be an effective technique to investigate the effect of pyrene on microorganisms. Copyright © 2010 Taylor & Francis Group, LLC.
|Journal||Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering|
|Publication status||Published - 2010|
CitationChen, Y., Yao, J., Chen, K., Wang, F., Zhou, Y., Chen, H., . . . Wong, M. H. (2010). Microcalorimetric investigation of the toxic action of pyrene on the growth of PAH-degrading bacteria Acinetobacter junii. Journal of Environmental Science and Health - Part A, 45(6), 668-673. doi: 10.1080/10934521003648826
- Toxicity measurement
- Acinetobacter junii
- Bacillus subtilis