Key internal factors leading to the variability in CO₂ fixation efficiency of different sulfur-oxidizing bacteria during autotrophic cultivation

Ya-nan WANG, Yan KAI, Lei WANG, Yiu Fai TSANG, Xiaohua FU, Jiajun HU, Yanjun XIE

Research output: Contribution to journalArticles

Abstract

Variability in the apparent CO₂ fixation yield of four aerobic sulfur-oxidizing bacteria (Halothiobacillus neapolitanus DSM 15147, Thiobacillus thioparus DSM 505, Thiomonas intermedia DSM 18155, and Starkeya novella DSM 506) in autotrophic culturing was studied, and mutual effects of key intrinsic factors on CO₂ fixation were explored. DSM 15147 and DSM 505 exhibited much higher CO₂ fixation yields than DSM 18155 and DSM 506. The differences in CO₂ fixation yield were determined not only by cbb gene transcription, but also by cell synthesis rate, which was determined by rRNA gene copy number; the rRNA gene copy number had a more significant effect than cbb gene transcription on the apparent CO₂ fixation yield. Moreover, accumulation of EDOC was observed in all four strains during chemoautotrophic cultivation, and the proportion of EDOC accounting for total fixed organic carbon (TOC; EDOC/TOC ratio) was much higher in DSM 18155 and DSM 506 than in DSM 15147 and DSM 505. The accumulation of EDOC led to a significant decrease in the cbb gene transcription efficiency during cultivation, and a further feedback inhibitory effect on CO₂ fixation. Copyright © 2020 Elsevier Ltd. All rights reserved.
Original languageEnglish
Article number110957
JournalJournal of Environmental Management
Volume271
Early online dateJun 2020
DOIs
Publication statusE-pub ahead of print - Jun 2020

Citation

Wang, Y.-N., Kai, Y., Wang, L., Tsang, Y. F., Fu, X., Hu, J., & Xie, Y. (2020). Key internal factors leading to the variability in CO₂ fixation efficiency of different sulfur-oxidizing bacteria during autotrophic cultivation. Journal of Environmental Management, 271. Retrieved from https://doi.org/10.1016/j.jenvman.2020.110957

Keywords

  • Inorganic carbon fixation
  • Sulfur-oxidizing bacteria (SOB)
  • Calvin-benson-bassham (CBB) cycle
  • cbb gene transcription efficiency
  • rRNA gene copy number
  • Extracellular dissolved organic carbon (EDOC)

Fingerprint Dive into the research topics of 'Key internal factors leading to the variability in CO₂ fixation efficiency of different sulfur-oxidizing bacteria during autotrophic cultivation'. Together they form a unique fingerprint.