Isolation and characterization of putative liver-specific enhancers in proboscis monkey (Nasalis larvatus)

Leonard Whye Kit LIM, Hung Hui CHUNG, Yee Ling CHONG, Nung Kion LEE

Research output: Contribution to journalArticle

Abstract

Enhancers are indispensable DNA elements responsible for elevation of gene transcriptional efficiency that regulates biological processes tightly at various developmental stages, linking them to numerous genetic diseases. Discovering the enhancer landscape of the genome will not only benefit mankind, but also aid in conservation researches involving endangered non-human primates such as the proboscis monkey. As one of the most ancient colobine endemic to Borneo Island, the proboscis monkey offers a wide spectrum of unique and exclusive characteristics that distinguish it from other primates. This study has successfully isolated 13 liver-specific enhancers from this primate and tested for their activities in HepG2 and A549 cell line. The TFBS-enriched regions such as pairs of AP-1, clusters of C/EBP-β and triplets of HNF-1 in enhancers contributed to enhancer activities whereas huge clusters of HNF-3β possess suppressing effects, but generally these regions contributed to the cell specificities of enhancers. It is hoped that this study serves as a stepping stone in knowledge enrichment on this primate and future conservation researches. Copyright © 2019 Universiti Putra Malaysia Press.
Original languageEnglish
Pages (from-to)627-647
JournalPertanika Journal of Tropical Agricultural Science
Volume42
Issue number2
Publication statusPublished - May 2019

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Primates
liver
genetic disorders
Borneo
cell lines
developmental stages
genome
Nasalis larvatus
DNA
genes
cells

Citation

Lim, L. W. K., Chung, H. H., Chong, Y. L., & Lee, N. K. (2019). Isolation and characterization of putative liver-specific enhancers in proboscis monkey (Nasalis larvatus). Pertanika Journal of Tropical Agricultural Science, 42(2), 627-647.

Keywords

  • Computational approach
  • Conservation
  • Enhancer
  • Liver
  • Proboscis monkey