Inhibition of CYP450scc expression in dioxin-exposed rat Leydig cells

K. P. LAI, Ming Hung WONG, C. K. C. WONG

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Abstract

Polychlorinated dibenzo-p-dioxins, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) have been recognized as highly potent developmental and reproductive toxins. We have previously demonstrated effects of TCDD in modulating the expression of rat Sertoli cell secretory products and markers for cell-cell interaction. In this study, we examined the direct biological effects of TCDD in rat Leydig cell primary cultures. Mature rat Leydig cells were purified by Percoll gradient centrifugation and the cell purity was determined by 3β-hydroxysteroid dehydrogenase (3β-HSD) staining and a testosterone induction assay. To examine TCDD-induced biological consequences, we measured the changes in the secretion of progesterone and testosterone, as well as transcript levels of some selected steroidogenic enzymes (i.e. StAR, P450scc, 3β-HSD and CYP17α), in TCDD/human chorionic gonadotropin (hCG) co-treated cells. Our results indicated that TCDD (0.2 or 2 ng/ml) treatment significantly suppressed hCG (5 or 10 ng/ml)-induced testosterone secretion. The suppressive effect aligned with a reduction of progesterone secretion (P<0.05), as well as a decrease of P450scc mRNA and protein expression (P<0.05). The mechanistic action of TCDD was found to be via the reduction of cellular cAMP levels in the hCG-treated cells. This observation was further confirmed, as the TCDD-mediated suppressive effect could be reversed by dibutyryl cAMP co-treatment. The data indicate that TCDD can modulate cAMP signaling in rat Leydig cells to affect the process of steroidogenesis. Copyright © 2005 Society for Endocrinology.

Original languageEnglish
Pages (from-to)519-527
JournalJournal of Endocrinology
Volume185
Issue number3
DOIs
Publication statusPublished - Jun 2005

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Dioxins
Leydig Cells
Chorionic Gonadotropin
3-Hydroxysteroid Dehydrogenases
Testosterone
Progesterone
Polychlorinated Dibenzodioxins
Steroid 17-alpha-Hydroxylase
Sertoli Cells
Centrifugation
Cell Communication
Cell Culture Techniques
Staining and Labeling
Messenger RNA

Citation

Lai, K. P., Wong, M. H., & Wong, C. K. C. (2005). Inhibition of CYP450scc expression in dioxin-exposed rat Leydig cells. Journal of Endocrinology, 185(3), 519-527. doi: 10.1677/joe.1.06054