Influence of reduced sulfur on carbon fixation efficiency of Halothiobacillus neapolitanus and its mechanism

Ya-nan WANG, Yiu Fai TSANG, Lei WANG, Xiaohua FU, Huan LI, Jiajun HU, Yiquan LE

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Abstract

The characteristics of carbon fixation of Halothiobacillus neapolitanus DSM 15147 with different concentrations of Na₂S₂O₃·5H₂O as reduced sulfur were investigated. Moreover, the mechanisms underlying the carbon fixation characteristics in response to different Na₂S₂O₃·5H₂O concentrations were clarified from the energy supply perspectives and the cbb gene transcription characteristics. The results showed that the carbon fixation efficiency of H. neapolitanus was influenced by the Na₂S₂O₃·5H₂O concentration, and the Na₂S₂O₃·5H₂O oxidization amount and energy-releasing efficiency per unit of Na₂S₂O₃·5H₂O were important reasons for the variation of carbon fixation efficiency at different Na₂S₂O₃·5H₂O concentrations. Moreover, the cbb gene transcription efficiency and transcription pattern were also influenced by the Na₂S₂O₃·5H₂O concentration, and when the Na₂S₂O₃·5H₂O concentration was too low or too high, it was not conducive to the cbb gene transcription process, thus resulting in the relatively low carbon fixation efficiency. Furthermore, low Na₂S₂O₃·5H₂O concentration was not beneficial to the cytoskeleton synthesis due to the insufficient energy supply and further resulted in the accumulation of extracellular free organic matters, which might induce a feedback inhibitory effect on the CO₂ assimilation process by influencing the cbb gene transcription efficiency. Copyright © 2017 Elsevier B.V.
Original languageEnglish
Pages (from-to)249-256
JournalChemical Engineering Journal
Volume326
Early online dateMay 2017
DOIs
Publication statusPublished - Oct 2017

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Citation

Wang, Y.-n., Tsang, Y. F., Wang, L., Fu, X., Li, H., Hu, J., et al. (2017). Influence of reduced sulfur on carbon fixation efficiency of Halothiobacillus neapolitanus and its mechanism. Chemical Engineering Journal, 326, 249-256.

Keywords

  • Halothiobacillus neapolitanus
  • Carbon fixation efficiency
  • Na₂S₂O₃·5H₂O concentration
  • Cbb gene transcription efficiency
  • Extracellular free organic matters