Industrial robustness linked to the gluconolactonase from Zymomonas mobilis

Alfonsus ALVIN, Junhyun KIM, Gwi-Taek JEONG, Yiu Fai TSANG, Eilhann E. KWON, Brett A. NEILAN, Young Jae JEON

Research output: Contribution to journalArticles

Abstract

The physiological characteristics and the potential gluconolactone production of the gluconolactonase-deficient strain, Zymomonas mobilis ZM₄ gnlΔ, were investigated via growth inhibitory assay and biotransformation of glucose and fructose into gluconolactone and sorbitol, respectively. The results of ethanol fermentation studies performed in the presence of high concentration of glucose (>200 g 1⁻¹) under fermentative or aerobic conditions indicated that a significant reduction of volumetric ethanol productivity from the strain of ZM₄ gnlΔ was noticeable due to the reduced rates of specific growth, sugar uptake, and biomass yield as compared with those of the parental strain ZM₄. The biotransformation prepared at pH 6.0 using the permeabilized cell indicated that gluconic acid from ZM₄ gnlΔ was still produced as a major product (67 g 1⁻¹) together with sorbitol (65 g 1⁻¹) rather than gluconolactone after 24 h. Only small amount of gluconolactone was transiently overproduced up to 9 g 1⁻¹, but at the end of biotransformation, all gluconolactone were oxidized into gluconic acid. This indicated that autolysis of gluconolactone at the pH led to such results despite under gluconolactonase inactivation conditions. The physiological characteristics of ZM₄ gnlΔ was further investigated under various stress conditions, including suboptimal pH (3.5~6.0), temperature (25~40 °C), and presence of growth inhibitory molecules including hydrogen peroxide, ethanol, acetic acid, furfural, and so forth. The results indicated that ZM₄ gnlΔ was more susceptible at high glucose concentration, low pH of 3.5, and high temperature of 40 °C and in the presence of 4 mM H₂O₂ comparing with ZM₄. Therefore, the results were evident that gluconolactonase in Z. mobilis contributed to industrial robustness and anti-stress regulation. Copyright © 2017 Springer-Verlag Berlin Heidelberg.
Original languageEnglish
Pages (from-to)5089-5099
JournalApplied Microbiology and Biotechnology
Volume101
Issue number12
Early online dateMar 2017
DOIs
Publication statusPublished - Jun 2017

Citation

Alvin, A., Kim, J., Jeong, G.-T., Tsang, Y. F., Kwon, E. E., Neilan, B. A., et al. (2017). Industrial robustness linked to the gluconolactonase from Zymomonas mobilis. Applied Microbiology and Biotechnology, 101(12), 5089-5099.

Keywords

  • Gene knockout
  • Gluconolactone
  • Gluconolactonase-deficient strain
  • Zymomonas mobilis
  • Robustness
  • Stress response

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