Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus)

Chunsheng LIU, Ke YU, Xiongjie SHI, Jingxian WANG, Paul K. S. LAM, Shiu Sun Rudolf WU, Bingsheng ZHOU

Research output: Contribution to journalArticles

213 Citations (Scopus)

Abstract

Perfluorinated organic compounds (PFOCs) are emerging persistent organic pollutants (POPs) widely present in the environment, wildlife and human. We studied the cellular toxicology of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) on oxidative stress and induction of apoptosis in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus). Cultured hepatocytes were exposed to PFOS or PFOA (0, 1, 5, 15 and 30 mg L-1) for 24 h, and a dose-dependent decrease in cell viability was determined using trypan blue exclusion method. Significant induction of reactive oxygen species (ROS) accompanied by increases in activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were found, while activities of glutathione peroxidase (GPx) and glutathione-S-transferase (GST) were decreased. Glutathione (GSH) content was reduced following treatment of PFOA and PFOS. A dose-dependent increase in the lipid peroxidation (LPO) level (measured as maleic dialdehyde, MDA) was observed only in the PFOA exposure groups, whereas LPO remained unchanged in the PFOS exposure groups. Furthermore, a significant activation of caspase-3, -8, -9 activities was evident in both PFOS and PFOA exposure groups. Typical DNA fragmentation (DNA laddering) was further characterized by agarose gel electrophoresis. The overall results demonstrated that PFOS and PFOA are able to produce oxidative stress and induce apoptosis with involvement of caspases in primary cultured tilapia hepatocytes. Copyright © 2007 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)135-143
JournalAquatic Toxicology
Volume82
Issue number2
DOIs
Publication statusPublished - May 2007

Citation

Liu, C., Yu, K., Shi, X., Wang, J., Lam, P. K. S., Wu, R. S. S., & Zhou, B. (2007). Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus). Aquatic Toxicology, 82(2), 135-143. doi: 10.1016/j.aquatox.2007.02.006

Keywords

  • Perfluorinated organic compounds
  • Primary cultured hepatocytes
  • Reactive oxygen species
  • Oxidative stress
  • Caspases
  • Apoptosis
  • Tilapia

Fingerprint Dive into the research topics of 'Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus)'. Together they form a unique fingerprint.