In situ hybridization to detect spatial gene expression in medaka

A. R. TOMPSETT, J. W. PARK, X. ZHANG, P. D. JONES, J. L. NEWSTED, D. W. T. AU, E. X. H. CHEN, R. YU, Shiu Sun Rudolf WU, R. Y. C. KONG, J. P. GIESY, M. HECKER

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9 Citations (Scopus)


A whole-animal tissue section in situ hybridization (ISH) system with radio-labeled probes was developed to detect differential gene expression among tissues of the small, oviparous teleost fish, Japanese medaka (Oryzias latipes). Because of its tissue- and gender-specific expression, gonadal aromatase (CYP19a) was selected as a model gene to demonstrate the potential of the system. The ISH system was validated with a 7 d exposure to the model aromatase inhibitor, fadrozole. Fadrozole did not affect the magnitude of gene expression in testes, but significantly up-regulated CYP19a gene expression in ovaries. These results were confirmed with quantitative real-time-polymerase chain reaction (RT-PCR). Histological evaluation revealed that females exposed to 100 μg/L fadrozole lacked mature oocytes. Male gonadal morphology was normal in all treatments. The ISH method developed in this study allowed tissue-specific resolution of gene expression in a whole animal model, as well as the ability to analyze cellular morphological detail in the same organism. Copyright © 2008 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)1257-1264
JournalEcotoxicology and Environmental Safety
Issue number4
Publication statusPublished - May 2009


Tompsett, A. R., Park, J. W., Zhang, X., Jones, P. D., Newsted, J. L., Au, D. W. T., . . . Hecker, M. (2009). In situ hybridization to detect spatial gene expression in medaka. Ecotoxicology and Environmental Safety, 72(4), 1257-1264. doi: 10.1016/j.ecoenv.2008.10.013


  • Histology
  • Fadrozole
  • Aromatase
  • CYP19
  • Hormones
  • Autoradiography
  • Oryzias latipes
  • RT-PCR
  • Gene expression
  • Endocrine disruption


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