Impairment of sea urchin sperm quality by UV-B radiation: Predicting fertilization success from sperm motility

D. W. T. AU, M. W. L. CHIANG, J. Y. M. TANG, B. B. H. YUEN, Y. L. WANG, Shiu Sun Rudolf WU

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67 Citations (Scopus)

Abstract

Sperm quality of the sea urchin, Anthocidaris crassispina, after exposure to environmentally realistic UV-B irradiances, was assessed by changes in sperm motility (measured by the computer-assisted sperm analysis (CASA) system), and related to subsequent fertilization success. Percentage motile sperm of A. crassispina declined significantly after exposure to a UV-B dose of 16.2 kJm-2, while sperm motion velocity as measured by curvilinear velocity (VCL), straight line velocity (VSL), and average path velocity (VAP) showed significant reduction after exposure to a UV-B dose of 5.4 kJm-2. A parallel study showed that fertilization success was significantly reduced after sperm were exposed to UV-B doses ≥5.4kJm-2. Notably, the four sperm motility parameters were strongly correlated with fertilization success (P<0.001), followed the increasing order: VSL (r=0.8)<%motilesperm(r=0.804)<VCL(r=0.912)<VAP(r=0.928). Fertilization success is best predicted by VAP using the exponential model: y=8.678+90.202/[1+exp(82.83-x)/10.27)] (r2=0.95). Thus, impairment of sperm motility of sea urchin, as measured by the CASA method, can be used to predict reproductive success and ecological effects. Copyright © 2002 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)583-589
JournalMarine Pollution Bulletin
Volume44
Issue number7
DOIs
Publication statusPublished - Jul 2002

Citation

Au, D. W. T., Chiang, M. W. L., Tang, J. Y. M., Yuen, B. B. H., Wang, Y. L., & Wu, R. S. S. (2002). Impairment of sea urchin sperm quality by UV-B radiation: Predicting fertilization success from sperm motility. Marine Pollution Bulletin, 44(7), 583-589. doi: 10.1016/S0025-326X(01)00288-0

Keywords

  • Sea urchin
  • Fertilization success
  • Sperm motility
  • UV-B radiation

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