Identification of a novel cytochrome P450 cDNA, CYP97E1, from the marine diatom Skeletonema costatum bacillariophyceae

Shao YANG, Shiu Sun Rudolf WU, Helen O. L. MOK, Z. P. ZHANG, Richard Y. C. KONG

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Abstract

Here we report identification of a 2269-base pair full-length cDNA, CYP97E1, encoding a novel cytochrome P450 protein from the marine diatom Skeletonema costatum. The CYP97E1 protein contains 659 amino acids (Mr 74,200) and is the largest P450 isoform described to date. Our BLAST homology search and parsimony analysis showed that CYP97E1 shared high sequence identity (>40%) and genetic relatedness, respectively, with the CYP97B isoforms from different plant species. CYP97E1 was predicted by PSORT (a protein localization site prediction program) to be a cytosolic protein. Northern hybridization analysis indicated that CYP97E1 expression in S. costatum was not significantly affected by 2,4-dichlorophenol, suggesting that CYP97E1 may not be involved in 2,4-dichlorophenol detoxification in this diatom. Copyright © 2003 Phycological Society of America.

Original languageEnglish
Pages (from-to)555-560
JournalJournal of Phycology
Volume39
Issue number3
DOIs
Publication statusPublished - Jun 2003

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Skeletonema costatum
Bacillariophyceae
cytochrome P-450
cytochrome
diatom
2,4-dichlorophenol
protein
proteins
parsimony analysis
detoxification
homology
relatedness
Northern blotting
genetic relationships
amino acid
amino acids
prediction

Citation

Yang, S., Wu, R. S. S., Mok, H. O. L., Zhang, Z. P., & Kong, R. Y. C. (2003). Identification of a novel cytochrome P450 cDNA, CYP97E1, from the marine diatom Skeletonema costatum bacillariophyceae. Journal of Phycology, 39(3), 555-560. doi: 10.1046/j.1529-8817.2003.02190.x

Keywords

  • Alga
  • Cytochrome P450
  • Diatom
  • Differential display
  • Rapid amplification of cDNA ends
  • Reverse transcription PCR
  • Skeletonema costatum