Here we report identification of a 2269-base pair full-length cDNA, CYP97E1, encoding a novel cytochrome P450 protein from the marine diatom Skeletonema costatum. The CYP97E1 protein contains 659 amino acids (Mr 74,200) and is the largest P450 isoform described to date. Our BLAST homology search and parsimony analysis showed that CYP97E1 shared high sequence identity (>40%) and genetic relatedness, respectively, with the CYP97B isoforms from different plant species. CYP97E1 was predicted by PSORT (a protein localization site prediction program) to be a cytosolic protein. Northern hybridization analysis indicated that CYP97E1 expression in S. costatum was not significantly affected by 2,4-dichlorophenol, suggesting that CYP97E1 may not be involved in 2,4-dichlorophenol detoxification in this diatom. Copyright © 2003 Phycological Society of America.
CitationYang, S., Wu, R. S. S., Mok, H. O. L., Zhang, Z. P., & Kong, R. Y. C. (2003). Identification of a novel cytochrome P450 cDNA, CYP97E1, from the marine diatom Skeletonema costatum bacillariophyceae. Journal of Phycology, 39(3), 555-560. doi: 10.1046/j.1529-8817.2003.02190.x
- Cytochrome P450
- Differential display
- Rapid amplification of cDNA ends
- Reverse transcription PCR
- Skeletonema costatum