Hypoxia-induced epigenetic transgenerational miRNAs dysregulation involved in reproductive impairment of ovary

Keng Po LAI, Cherry Chi Tim LEUNG, Delbert Almerick T. BONCAN, Nathan TAM, Xiao LIN, Simon Yuan WANG, Ting Fung CHAN, Shiu Sun Rudolf WU, Richard Yuen Chong KONG

Research output: Contribution to journalArticlespeer-review

Abstract

Hypoxia is a potent endocrine disruptor that is posing serious problems to the fish reproductive systems. Our previous studies reported that hypoxia could cause a transgenerational impairment of ovarian development and interfere hatching success in F2 offspring of marine medaka fish (Oryzias melastigma) through epigenetic regulation. As part of the epigenetic regulation, we investigated the involvement of microRNAs (miRNAs) in hypoxia-induced transgenerational reproductive impairments. In the present study, we used comparative small RNA sequencing to reveal that hypoxia caused miRNA dysregulation in ovaries of F0 hypoxia group and F2 transgenerational group. We found 4 common dysregulated miRNA in the F0 and F2 generations. Furthermore, integrated miRNA-mRNA analysis, followed by gene ontology enrichment analysis on the hypoxia-dysregulated miRNA-target genes further highlighted the importance of these dysregulated miRNAs in biological processes related to reproduction. More importantly, we identified 3 miRNA-mRNA pairs (novel miRNA-525-DIAPH2, novel miRNA-525-MYOCD, and novel miRNA-525-RAI14) that might play epigenetic roles in hypoxia-induced reproductive impairment. For the first time, our findings suggested the involvement of miRNA in hypoxia-induced reproductive impairments may be inherited via a transgenerational manner. Copyright © 2022 Elsevier B.V. All rights reserved.

Original languageEnglish
Article number110176
JournalChemico-Biological Interactions
Volume367
Early online dateSep 2022
DOIs
Publication statusPublished - Nov 2022

Citation

Lai, K. P., Leung, C. C. T., Boncan, D. A. T., Tam, N., Lin, X., Wang, S. Y., . . . Kong, R. Y. C. (2022). Hypoxia-induced epigenetic transgenerational miRNAs dysregulation involved in reproductive impairment of ovary. Chemico-Biological Interactions, 367. Retrieved from https://doi.org/10.1016/j.cbi.2022.110176

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