Growth-differentiation factor-8 (GDF-8) in the uterus: Its identification and functional significance in the golden hamster

Chun Lung WONG, Ya Yu HUANG, Wing Kei HO, Hong Kit POON, Pui Lai CHEUNG, Wai Sum O, Pak Ham CHOW

Research output: Contribution to journalArticles

3 Citations (Scopus)

Abstract

Transforming growth factor-beta superfamily regulates many aspects of reproduction in the female. We identified a novel member of this family, growth-differentiation factor 8 (GDF-8) in the 72 h post coital uterine fluid of the golden hamster by proteomic techniques. Uterine GDF-8 mRNA decreased as pregnancy progressed while its active protein peaked at 72 h post coitus (hpc) and thereafter stayed at a lower level. At 72 hpc, the GDF-8 transcript was localized to the endometrial epithelium while its protein accumulated in the stroma. Exogenous GDF-8 slowed down proliferation of primary cultures of uterine smooth muscle cells (SMC) and endometrial epithelial cells (EEC). In addition, GDF-8 attenuated the release of LIF (leukaemia inhibiting factor) by EEC. As for the embryo in culture, GDF-8 promoted proliferation of the trophotoderm (TM) and hatching but discouraged attachment. Our study suggests that GDF-8 could regulate the behavior of preimplantation embryos and fine-tune the physiology of uterine environment during pregnancy. Copyright © 2009 Wong et al; licensee BioMed Central Ltd.

Original languageEnglish
Article number134
JournalReproductive Biology and Endocrinology
Volume7
DOIs
Publication statusPublished - 25 Nov 2009

Citation

Wong, C. L., Huang, Y. Y., Ho, W. K., Poon, H. K., Cheung, P. L., O, W. S. & Chow, P. H. (2009). Growth-differentiation factor-8 (GDF-8) in the uterus: Its identification and functional significance in the golden hamster. Reproductive Biology and Endocrinology, 7. Retrieved from https://doi.org/10.1186/1477-7827-7-134

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