Global picture of protein regulation in response to dibutyl phthalate (DBP) stress of two Brassica parachinensis cultivars differing in DBP accumulation

Hai-Ming ZHAO, He-Biao HUANG, Huan DU, Lei XIANG, Ce-Hui MO, Yan-Wen LI, Quan-Ying CAI, Hui LI, Jie-Sheng LIU, Dong-Mei ZHOU, Ming Hung WONG

Research output: Contribution to journalArticles

9 Citations (Scopus)

Abstract

iTRAQ analysis was used to map the proteomes of two Brassica parachinensis cultivars that differed in dibutyl phthalate (DBP) accumulation. A total of 5699 proteins were identified to obtain 152 differentially regulated proteins, of which 64 and 48 were specific to a high- and a low-DBP-accumulation cultivar, respectively. Genotype-specific biological processes were involved in coping with DBP stress, accounting for the variation in DBP tolerance and accumulation. Formation of high DBP accumulation in B. parachinensis might attribute to the more effective regulation of protein expression in physiology and metabolism, including (a) enhanced cell wall biosynthesis and modification, (b) better maintenance of photosynthesis and energy balance, (c) greatly improved total capacity for antioxidation and detoxification, and (d) enhanced cellular transport and signal transduction. Our novel findings contribute to a global picture of DBP-induced alterations of protein profiles in crops and provide valuable information for the development of molecular-assisted breeds of low-accumulation cultivars. Copyright © 2018 American Chemical Society.
Original languageEnglish
Pages (from-to)4768-4779
JournalJournal of Agricultural and Food Chemistry
Volume66
Issue number18
Early online dateApr 2018
DOIs
Publication statusPublished - 2018

Citation

Zhao, H.-M., Huang, H.-B., Du, H., Xiang, L., Mo, C.-H., Li, Y.-W., . . . Wong, M.-H. (2018). Global picture of protein regulation in response to dibutyl phthalate (DBP) stress of two Brassica parachinensis cultivars differing in DBP accumulation. Journal of Agricultural and Food Chemistry, 66(18), 4768-4779. doi: 10.1021/acs.jafc.8b01157

Keywords

  • Accumulation variation
  • Brassica parachinensis
  • Dibutyl phthalate (DBP) stress
  • Low-accumulation cultivars
  • Proteomic

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