Generation of reactive oxygen species and oxidative stress in Escherichia coli and Staphylococcus aureus by a novel semiconductor catalyst

K. L. CHOW, N. K. MAK, Ming Hung WONG, X. F. ZHOU, Y. LIANG

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10 Citations (Scopus)

Abstract

The objective of this study was to investigate antimicrobial mechanisms of a new catalytic material (charge transfer auto oxidation-reduction type catalyst, CT catalyst) that may have great potential for application in water/wastewater treatment. Generation of reactive oxygen species (ROS) in bacteria-free solution, induction of ROS and oxidative damage in bacteria (including E. coli and S. aureus) were examined for the CT catalyst. The results showed that significantly higher (p < 0.05, via t-test) amount of hydroxyl radicals was generated by the CT catalyst compared with the control, particularly after 6 h of contact time that more than twice of the amount of the control was produced. The generation of ROS in the bacteria was greater under higher pH and temperature levels, which closely related with the oxidative damage in cells. The results indicated that CT catalyst induced oxidative damage in the bacteria might serve as an important mechanism interpreting the anti-microbial function of the CT catalyst. Copyright © 2010 Springer Science+Business Media B.V.

Original languageEnglish
Pages (from-to)1007-1017
JournalJournal of Nanoparticle Research
Volume13
Issue number3
DOIs
Publication statusPublished - Mar 2011

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Oxidative Stress
Reactive Oxygen Species
Oxidative stress
staphylococcus
Escherichia
Catalyst
Escherichia coli
Escherichia Coli
Semiconductors
Semiconductor materials
catalysts
Bacteria
bacteria
Catalysts
Oxygen
oxygen
Damage
damage
Wastewater Treatment
water treatment

Citation

Chow, K. L., Mak, N. K., Wong, M. H., Zhou, X. F., & Liang, Y. (2011). Generation of reactive oxygen species and oxidative stress in Escherichia coli and Staphylococcus aureus by a novel semiconductor catalyst. Journal of Nanoparticle Research, 13(3), 1007-1017. doi: 10.1007/s11051-010-0128-7

Keywords

  • Superoxide anions
  • Hydroxyl radicals
  • Lipid peroxidation
  • Protein oxidation
  • Antibacterial activity
  • Charge transfer auto oxidation–reduction type catalyst