Gender-specific transcriptional profiling of marine medaka (Oryzias melastigma) liver upon BDE-47 exposure

W. K. YU, Y. F. SHI, C. C. FONG, Y. CHEN, J. P. VAN DE MERWE, Alice K. Y. CHAN, F. WEI, J. BO, R. YE, Doris W. T. AU, Shiu Sun Rudolf WU, M. S. YANG

Research output: Contribution to journalArticles

14 Citations (Scopus)

Abstract

Marine medaka (Oryzias melastigma) were exposed to 2,2′,4,4′- tetrabromodiphenyl ether (BDE-47) to investigate the gender-specific transcriptional profiles of liver tissue in response to this flame retardant. A cDNA library of O. melastigma was constructed, and 2304 clones were amplified from the library to fabricate a cDNA microarray. Sequences of these genes were assembled into 1800 sequences using Geneious, a bioinformatics software. Corresponding expressed sequence tags were blasted against the National Centre for Biotechnology Information non-redundant database and further classified into various biological categories according to the Gene Ontology project. Male and female three-month-old were fed a diet of BDE-47 contaminated Artemia at low dosage (290.3 ± 172.3 ng BDE-47/day) and high dosage (580.5 ± 344.6 ng BDE-47/day) for 5 and 21 days, respectively. The transcriptional profiles of O. melastigma liver were then generated by the species-specific cDNA microrarray. The results from microarray analysis suggested very different gene expression patterns between males and females for both BDE-47 exposure-dose and exposure-time, with male livers having stronger gene regulatory responses than female livers. Importantly, our results revealed that in male O. melastigma only, BDE-47 exposure may activate phosphoinositide-3-kinase and mitogen-activated protein kinase, proteins that play importance roles in cell growth, proliferation and survival. Copyright © 2013 Published by Elsevier B.V.

Original languageEnglish
Pages (from-to)255-262
JournalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume8
Issue number3
Early online date04 Jul 2013
DOIs
Publication statusPublished - Sep 2013

Citation

Yu, W. K., Shi, Y. F., Fong, C. C., Chen, Y., van de Merwe, J. P., Chan, A. K. Y., . . . Yang, M. S. (2013). Gender-specific transcriptional profiling of marine medaka (Oryzias melastigma) liver upon BDE-47 exposure. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 8(3), 255-262. doi: 10.1016/j.cbd.2013.06.004

Keywords

  • Oryzias melastigma
  • BDE-47
  • -omics

Fingerprint Dive into the research topics of 'Gender-specific transcriptional profiling of marine medaka (Oryzias melastigma) liver upon BDE-47 exposure'. Together they form a unique fingerprint.