Marine medaka (Oryzias melastigma) were exposed to 2,2′,4,4′- tetrabromodiphenyl ether (BDE-47) to investigate the gender-specific transcriptional profiles of liver tissue in response to this flame retardant. A cDNA library of O. melastigma was constructed, and 2304 clones were amplified from the library to fabricate a cDNA microarray. Sequences of these genes were assembled into 1800 sequences using Geneious, a bioinformatics software. Corresponding expressed sequence tags were blasted against the National Centre for Biotechnology Information non-redundant database and further classified into various biological categories according to the Gene Ontology project. Male and female three-month-old were fed a diet of BDE-47 contaminated Artemia at low dosage (290.3 ± 172.3 ng BDE-47/day) and high dosage (580.5 ± 344.6 ng BDE-47/day) for 5 and 21 days, respectively. The transcriptional profiles of O. melastigma liver were then generated by the species-specific cDNA microrarray. The results from microarray analysis suggested very different gene expression patterns between males and females for both BDE-47 exposure-dose and exposure-time, with male livers having stronger gene regulatory responses than female livers. Importantly, our results revealed that in male O. melastigma only, BDE-47 exposure may activate phosphoinositide-3-kinase and mitogen-activated protein kinase, proteins that play importance roles in cell growth, proliferation and survival. Copyright © 2013 Published by Elsevier B.V.
|Journal||Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics|
|Early online date||04 Jul 2013|
|Publication status||Published - Sep 2013|
CitationYu, W. K., Shi, Y. F., Fong, C. C., Chen, Y., van de Merwe, J. P., Chan, A. K. Y., . . . Yang, M. S. (2013). Gender-specific transcriptional profiling of marine medaka (Oryzias melastigma) liver upon BDE-47 exposure. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 8(3), 255-262. doi: 10.1016/j.cbd.2013.06.004
- Oryzias melastigma