Fish and crustacean biodiversity in an outer maritime estuary of the Pearl River Delta revealed by environmental DNA

Chi Chiu CHEANG, Bo Yee LEE, Ho Yeung Brian IP, Wai Hong YIU, Ling-ming TSANG, Put O. Jr ANG

Research output: Contribution to journalArticlespeer-review

Abstract

Understanding the faunal community structure in the estuary would be crucial in assessing the health of the ecosystem. The poor visibility in the estuarine area due to the outflow from the Pearl River hinders the conventional visual census in assessing the megafaunal biodiversity. In this study, the fish and crustacean biodiversity of Hong Kong's western waters, i.e. the outer maritime estuary of the PRD, were studied through the metabarcoding of environmental DNA (eDNA). eDNA from the seawater and sediment samples was extracted from five sites in the region. After testing the performance of two genetic markers, amplicons of the cytochrome oxidase I, amplified by polymerase chain reaction, were subjected to Illumina high-throughput sequencing (MiSeq) analysis. A total of 22 fish species from 17 families and 34 crustacean species from 27 families were identified by blasting the sequences against the NCBI GenBank database, demonstrating segregation between samples from different sites. This study provides insight on the detail distribution of fish assembly in PRD, when compared with a previous eDNA study in the inner brackish PRD. Copyright © 2020 Elsevier Ltd. All rights reserved.
Original languageEnglish
Article number11707
JournalMarine Pollution Bulletin
Volume161
Issue numberPt. A
Early online date13 Oct 2020
DOIs
Publication statusE-pub ahead of print - 13 Oct 2020

Citation

Cheang, C.-C., Lee, B.-Y., Ip, B. H.-Y., Yiu, W.-H., Tsang, L.-M., & Ang, P. O., Jr. (2020). Fish and crustacean biodiversity in an outer maritime estuary of the Pearl River Delta revealed by environmental DNA. Marine Pollution Bulletin, 161(Pt. A). Retrieved from https://doi.org/10.1016/j.marpolbul.2020.111707

Keywords

  • Cytochrome oxidase I (COI)
  • High-throughput sequencing
  • Marine
  • Metabarcoding
  • Subtropical

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