Feasibility of Sijunzi Tang (Chinese medicine) to enhance protein disulfide isomerase activities for reactivating malate dehydrogenase deactivated by polycyclic aromatic hydrocarbons

Ho Man LEUNG, Francis Siu Lai KWOK, Wing Yin MO, Kwai Chung CHEUNG, Yik Kit YUE, Yee Keung WONG, Chi Kin AU, Martin Tsz Ki TSUI, Ken Kin Lam YUNG

Research output: Contribution to journalArticles

Abstract

The objective of this research is to investigate the enzymatic activities between protein disulfide isomerase (PDI) found in animals and plants and the properties found in a commonly used Chinese medicine called Sijunzi Tang. During the investigation, PDI, which is a monomer with a molecular mass of 57.0 kDa, was used to reactivate malate dehydrogenase (MDH). However, with the interference of polycyclic aromatic hydrocarbons (PAHs), evidence indicates that such chemicals are carcinogenic, mutagenic, and toxic to humans. The enzymatic activity of PDI found in animal’s liver and plant was 1657 folds of purification; 0.284 unit/mg of enzyme activity, and 5694.4 folds of purification; 1.00 unit/mg of enzyme activity, respectively. PDI extracted in treated animal and plant tissue revealed 2.40% and 80.44% of regaining MDH enzymatic activity, respectively. Although in its initial phase of investigation, it is assumed that the properties found in Sijunzi Tang can help regain enzymatic activity in those affected by xenobiotic substances, thus, making it a potential ingredient in assisting with PDI functions. Copyright © 2018 Springer-Verlag GmbH Germany, part of Springer Nature.
Original languageEnglish
JournalEnvironmental Science and Pollution Research
Early online dateOct 2018
DOIs
Publication statusE-pub ahead of print - Oct 2018

Citation

Leung, H. M., Kwok, F. S. L., Mo, W. Y., Cheung, K. C., Yue, Y. K., Wong, Y. K., . . . Yung, K. K. L. (2018). Feasibility of Sijunzi Tang (Chinese medicine) to enhance protein disulfide isomerase activities for reactivating malate dehydrogenase deactivated by polycyclic aromatic hydrocarbons. Environmental Science and Pollution Research. Advance online publication. doi: 10.1007/s11356-018-3230-5

Keywords

  • Protein disulfide isomerase (PDI)
  • Malate dehydrogenase (MDH)
  • Sijunzi Tang
  • Glutathione reductase assay
  • Polycyclic aromatic hydrocarbons (PAHs)

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