Faunistic and DNA barcoding of the recorded spiders (Araneae: Gnaphosidae, Hersiliidae and Salticidae) from Lower Dir (Pakistan)

Spiders of family Gnaphsidae Pocock (1898), Hersiliidae Thorell (1870) and Salticidae Blackwall (1841) were collected from Dir Lower Pakistan and were preserved in ethanol. Preserved specimens were studied under stereomicroscope and photographed with the help of camera mounted on compound microscope. New faunistic records for three species Micaria dives Lucas (1846), Hersilia savignyi Lucas (1836) and Cyrba ocellata Kroneberg (1875) are provided from the country with brief description, digital photographs of external morphology as well as genitalia. While an increased distribution ranges of three species Plexippus paykulli Audouin (1826), Menemerus nigli Wesolowska and Freudenschuss (2012) and Thyene imperialis Rossi (1846) that is already reported from Pakistan. DNA base identification for all species is provided. COI sequences >200 bp recovered from the specimens were analyzed using neighbor-joining trees and Barcode Index Numbers (BINs) Nucleotide alignment for each species is provided with Process id, Sample id, match BIN and match process id. Arc GIS (10.5) was used to show the distribution map of the species in various localities. This study will impel and will help the taxonomist for future work on unknown biodiversity of Pakistan. Copyright © 2022 Friends Science Publishers.