Extract of Zuojin Pill (左金丸) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway

Qiu-xian PENG, Hong-bing CAI, Jiang-li PENG, Kin Lam Ken YUNG, Jue SHI, Zhi-xian MO

Research output: Contribution to journalArticlespeer-review

5 Citations (Scopus)

Abstract

Objective: To observe the effects of water extract of Zuojin Pill (左金丸, ZJP) on inhibiting the growth of human gastric cancer cell line SGC-7901 and its potential mechanism. Methods: Effects of ZJP on SGC-7901 cells growth were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, cell apoptosis and cell cycle were determined by flow cytometry, and apoptosis induction was detected by means of DNA gel electrophoresis. The cellular mechanism of drug-induced cell death was unraveled by assaying oxidative injury level of SGC-7901 cell, mitochondrial membrane potentials, expression of apoptosis-related genes, such as B cell lymphoma/lewkmia-2 (Bcl-2), Bcl-2 associated X protein (Bax) and cleaved caspase-3 and caspase-9. Results: ZJP exerted evident inhibitory effect on SGC-7901 cells by activating production of reactive oxygen species and elevating Bax/Bcl-2 ratio in SGC-7901 cells, leading to attenuation of mitochondrial membrane potential and DNA fragmentation. Conclusions: ZJP inhibits the cancer cell growth via activating mitochondria-dependent apoptosis pathway. ZJP can potentially serve as an antitumor agent. Copyright © 2015 Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg.

Original languageEnglish
Pages (from-to)837-845
JournalChinese Journal of Integrative Medicine
Volume21
Early online dateApr 2015
DOIs
Publication statusPublished - Nov 2015

Citation

Peng, Q.-X., Cai, H.-B., Peng, J.-L., Yung, K. L., Shi, J., & Mo, Z.-X. (2015). Extract of Zuojin Pill (左金丸) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway. Chinese Journal of Integrative Medicine, 21, 837-845. https://doi.org/10.1007/s11655-015-2043-3

Keywords

  • Zuojin Pill
  • Mitochondria-dependent pathway
  • Apoptosis
  • Reactive oxygen species
  • Chinese medicine

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