Evolutionary mode of metallothioneins inferred from Cd-MT genes of Tetrahymena

Ming-liang WAN, Wei-song FENG, Wei MIAO, Ming Hung WONG

Research output: Contribution to journalArticlespeer-review

2 Citations (Scopus)


From Tetrahymena thermophila (strain BF5), the coding region ofCd-MT gene was cloned and sequenced, and identified as MTTI isoform. A serial duplicate structure is discovered in its amino acid sequence, which separates the coding region into three parts (Part 1: 7–61: Part 2: 64–118; Part 3: 122–162). The alignments among them and comparison with the corresponding parts of MT1 isoform suggest that MT1 and MTT1 isoforms both come from the same ancient gene that is homologous to Part 1, and Cd-MTs of Tetrahymena are aroused by such ancient gene duplication. The prediction of secondary structures and the analysis of the disulfide-bonding state of cysteine show that there are a lot of differences between MT1 and MTT1 isoforms, which maybe relate to their function mechanism. Copyright © 2005 Wuhan University.
Original languageEnglish
Pages (from-to)925-930
JournalWuhan University Journal of Natural Sciences
Issue number5
Publication statusPublished - Sept 2005


Wan, M.-L., Feng, W.-S., Miao, W., & Wong, M.-H. (2005). Evolutionary mode of metallothioneins inferred from Cd-MT genes of Tetrahymena. Wuhan University Journal of Natural Sciences, 10(5), 925-930. doi: 10.1007/BF02832440


  • Tetrahymena thermophila
  • Metallothioneins
  • Evolutionary mode
  • Gene duplication
  • Protein spatial structure
  • Metal-binding sites