Evaluation of genotoxic and mutagenic activity of organic extracts from drinking water sources

Ying GUAN, Xiaodong WANG, Ming Hung WONG, Guoping SUN, Taicheng AN, Jun GUO, Guoxia ZHANG

Research output: Contribution to journalArticles

8 Citations (Scopus)

Abstract

An increasing number of industrial, agricultural and commercial chemicals in the aquatic environment lead to various deleterious effects on organisms, which is becoming a serious global health concern. In this study, the Ames test and SOS/umu test were conducted to investigate the potential genotoxicity and mutagenicity caused by organic extracts from drinking water sources. Organic content of source water was extracted with XAD-2 resin column and organic solvents. Four doses of the extract equivalent to 0.25, 0.5, 1 and 2L of source water were tested for toxicity. All the water samples were collected from six different locations in Guangdong province. The results of the Ames test and SOS/umu test showed that all the organic extracts from the water samples could induce different levels of DNA damage and mutagenic potentials at the dose of 2 L in the absence of S9 mix, which demonstrated the existence of genotoxicity and mutagenicity. Additionally, we found that Salmonella typhimurium strain TA98 was more sensitive for the mutagen. Correlation analysis between genotoxicity, Organochlorine Pesticides (OCPs) and Polycyclic Aromatic Hydrocarbons (PAHs) showed that most individual OCPs were frame shift toxicants in drinking water sources, and there was no correlation with total OCPs and PAHs. Copyright © 2017 Guan et al.
Original languageEnglish
Article numbere0170454
JournalPLoS One
Volume12
Issue number1
DOIs
Publication statusPublished - Jan 2017

Citation

Guan, Y., Wang, X., Wong, M., Sun, G., An, T., Guo, J., & Zhang, G. (2017).Evaluation of genotoxic and mutagenic activity of organic extracts from drinking water sources. PLoS One, 12(1). Retrieved from https://doi.org/10.1371/journal.pone.0170454

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