Enhanced dissipation of DEHP in soil and simultaneously reduced bioaccumulation of DEHP in vegetable using bioaugmentation with exogenous bacteria

Hai-Ming ZHAO, Rui-Wen HU, He-Biao HUANG, Hong-Fei WEN, Huan DU, Yan-Wen LI, Hui LI, Quan-Ying CAI, Ce-Hui MO, Jie-Sheng LIU, Ming Hung WONG

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19 Citations (Scopus)

Abstract

The widely used plastic film containing di(2-ethylhexyl) phthalate (DEHP) in agriculture has caused serious soil pollution and poses risks to human health through the food chain. An effective DEHP degradation bacteria, Microbacterium sp. J-1, was newly isolated from landfill soil. Response surface methodology was successfully employed for optimization resulting in 96% degradation of DEHP (200 mg L⁻¹) within 5 days. This strain degraded DEHP by hydrolysis of the ester bond and hydroxylation of the aromatic ring to form 2-ethyl hexanol, mono-(2-ethylhexyl) phthalate, phthalate acid, and protocatechuic acid, and subsequently transformed these compounds with a maximum specific degradation rate (qmax), half-saturation constant (Ks), and inhibition constant (Ki) of 1.46 day⁻¹, 180.2 mg L⁻¹, and 332.8 mg L⁻¹, respectively. Bioaugmentation of DEHP-contaminated soils with the strain J-1 greatly enhanced the DEHP dissipation rate (~88%). Moreover, this strain could efficiently colonize the rhizosphere soil of inoculated vegetables and further enhanced DEHP degradation (~97%), leading to a significant decrease (>70%) in DEHP accumulation in shoots and roots of the inoculated vegetables compared to uninoculated vegetables. The results highlighted the roles of the inoculated exogenous bacteria in simultaneously bioremediating contaminated soils and reducing bioaccumulation of DEHP in the edible part of the vegetable for food safety. Copyright © 2017 Springer-Verlag Berlin Heidelberg.
Original languageEnglish
Pages (from-to)663-675
JournalBiology and Fertility of Soils
Volume53
Issue number6
Early online dateMay 2017
DOIs
Publication statusPublished - Aug 2017

Citation

Zhao, H.-M., Hu, R.-W., Huang, H.-B., Wen, H.-F., Du, H., Li, Y.-W., et al. (2017). Enhanced dissipation of DEHP in soil and simultaneously reduced bioaccumulation of DEHP in vegetable using bioaugmentation with exogenous bacteria. Biology and Fertility of Soils, 53(6), 663–675.

Keywords

  • Phthalate esters (PAEs)
  • Microbacterium sp. J-1
  • Degradation pathway
  • Soil bioremediation
  • Vegetable accumulation
  • PCR-DGGE

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