Endothelin type B receptor promotes cofilin rod formation and dendritic loss in neurons by inducing oxidative stress and cofilin activation

Sze-Wah TAM, Rui FENG, Kwok Wai LAU, Andrew Chi-Kin LAW, Patrick Ka-Kit YEUNG, Sookja Kim CHUNG

Research output: Contribution to journalArticles

Abstract

Endothelin-1 (ET-1) is a neuroactive peptide produced by neurons, reactive astrocytes, and endothelial cells in the brain. Elevated levels of ET-1 have been detected in the post-mortem brains of individuals with Alzheimer's disease (AD). We have previously demonstrated that overexpression of astrocytic ET-1 exacerbates memory deficits in aged mice or in APPK670/M671 mutant mice. However, the effects of ET-1 on neuronal dysfunction remain elusive. ET-1 has been reported to mediate superoxide formation in the vascular system via NADPH oxidase (NOX) and to regulate the actin cytoskeleton of cancer cell lines via the cofilin pathway. Interestingly, oxidative stress and cofilin activation were both reported to mediate one of the AD histopathologies, cofilin rod formation in neurons. This raises the possibility that ET-1 mediates neurodegeneration via oxidative stress– or cofilin activation–driven cofilin rod formation. Here, we demonstrate that exposure to 100 nM ET-1 or to a selective ET type B receptor (ETB) agonist (IRL1620) induces cofilin rod formation in dendrites of primary hippocampal neurons, accompanied by a loss of distal dendrites and a reduction in dendritic length. The 100 nM IRL1620 exposure induced superoxide formation and cofilin activation, which were abolished by pretreatment with a NOX inhibitor (5 μM VAS2870). Moreover, IRL1620-induced cofilin rod formation was partially abolished by pretreatment with a calcineurin inhibitor (100 nM FK506), which suppressed cofilin activation. In conclusion, our findings suggest a role for ETB in neurodegeneration by promoting cofilin rod formation and dendritic loss via NOX-driven superoxide formation and cofilin activation. Copyright © 2019 Tam et al.
Original languageEnglish
Pages (from-to)12495-12506
JournalJournal of Biological Chemistry
Volume294
Issue number33
Early online dateJun 2019
DOIs
Publication statusPublished - 2019

Citation

Tam, S.-W., Feng, R., Lau, W. K.-W., Law, A. C.-K., Yeung, P. K.-K., & Chung, S. K. (2019). Endothelin type B receptor promotes cofilin rod formation and dendritic loss in neurons by inducing oxidative stress and cofilin activation. Journal of Biological Chemistry, 294(33), 12495-12506. doi: 10.1074/jbc.RA118.005155

Keywords

  • Cofilin
  • Dendrite
  • NADPH oxidase
  • Oxidative stress
  • Neurodegeneration
  • Alzheimer's disease
  • Endothelin type B receptor
  • Endothelin-1

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