Effects of zinc (zinc sulfate) on Rhizobia-earleaf acacia (Acacia auriculaeformis) symbiotic association

Z.Q. ZHANG, Ming Hung WONG, X.P. NIE, C.Y. LAN

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This project was aimed at investigating the feasibility of using a legume woody plant Acacia auriculaeformis to reclaim Zn-contaminated areas. The effects of different concentrations of Zn, applied as zinc sulfate, on nodulation (%) and nitrogen fixation activity of the A. auriculaeformis symbiont Rhizobium strain (AA9108) and on growth of the host plant in solution culture conditions were studied. The EC₁₀ and EC₅₀ values (effective concentration to reduce the growth by 10% and 50%, respectively) for AA9108 were 373.6 mg l⁻¹ and 638.9 mg l⁻¹, respectively. Nodule formation was inhibited by 10% and 50% at Zn levels of 62.9 mg l⁻¹ and 115.6 mg l⁻¹, respectively. EC₁₀ and EC₅₀ values for N₂-fixation activity were 4.7 mg l⁻¹ and 38.6 mg l⁻¹, respectively. Growth of host plant was the most sensitive (EC₁₀ and EC₅₀ values were 0.6 mg l⁻¹ and 18.3 mg l⁻¹, respectively). Screening Zn-tolerant varieties of the genus Acacia is therefore a prerequisite for using this genus in revegetation of Zn-contaminated areas. Copyright © 1998 Published by Elsevier Ltd.
Original languageEnglish
Pages (from-to)97-104
JournalBioresource Technology
Issue number2
Publication statusPublished - May 1998



Zhang, Z. Q., Wong, M. H., Nie, X. P., & Lan, C. Y. (1998). Effects of zinc (zinc sulfate) on Rhizobia-earleaf acacia (Acacia auriculaeformis) symbiotic association. Bioresource Technology, 64(2), 97-104. doi: 10.1016/S0960-8524(97)00183-1


  • Legume
  • Mine tailings
  • Nitrogen-fixation
  • Nodulation
  • Revegetation