Effects of nutrients, salinity, pH and light: Dark cycle on the production of reactive oxygen species in the alga Chattonella marina

Wenhua LIU, Doris W. T. AU, Donald M. ANDERSON, Paul K. S. LAM, Shiu Sun Rudolf WU

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Abstract

Experiments were carried out to investigate the effects of nutrients, salinity, pH and light:dark cycle on growth rate and production of reactive oxygen species (ROS) by Chattonella marina, a harmful algal bloom (HAB) species that often causes fish kills. Different nitrogen forms (organic-N and inorganic-N), N:P ratios, light:dark cycles and salinity significantly influenced algal growth, but not ROS production. However, iron concentration and pH significantly affected both growth and ROS production in C. marina. KCN (an inhibitor of mitochondrial respiration) and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (an inhibitor of photosynthesis) had no significant effects on ROS production. Vitamin K3 (a plasma membrane electron shuttle) enhanced ROS production while its antagonist, dicumarol, decreased ROS production. Taken together, our results suggest that ROS production by C. marina is related to a plasma membrane enzyme system regulated by iron availability but is independent of growth, photosynthesis, availability of macronutrients, salinity and irradiance. Copyright © 2007 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)76-86
JournalJournal of Experimental Marine Biology and Ecology
Volume346
Issue number1-2
DOIs
Publication statusPublished - 03 Aug 2007

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Citation

Liu, W., Au, D. W. T., Anderson, D. M., Lam, P. K. S., & Wu, R. S. S. (2007). Effects of nutrients, salinity, pH and light: Dark cycle on the production of reactive oxygen species in the alga Chattonella marina. Journal of Experimental Marine Biology and Ecology, 346(1-2), 76-86. doi: 10.1016/j.jembe.2007.03.007

Keywords

  • Chattonella marina
  • Iron
  • Nutrient
  • pH
  • Physical parameters
  • Plasma membrane
  • Reactive oxygen species
  • Redox