Effects of arbuscular mycorrhizal fungi on redox homeostasis of rice under Cd stress

Hui LI, Xun Wen CHEN, Li WU, Na LUO, Wei Xiong HUANG, Ce Hui MO, Yan Wen LI, Lei XIANG, Hai Ming ZHAO, Quan Ying CAI, Ming Hung WONG

Research output: Contribution to journalArticles

Abstract

Aims  Arbuscular mycorrhizal fungi (AMF) can enhance cadmium (Cd) tolerance and decrease Cd uptake of rice plant. However, in these processes, the essential role of reactive oxygen species (ROS)-antioxidants interactions is so far not investigated.
Methods  We inoculated AMF, Funneliformis mosseae (Fm) and Rhizophagus intraradices (Ri), to upland rice subjected to 0, 2 or 10 mg Cd kg⁻¹ in soil. ROS, antioxidants, chlorophyll, mesophyll cell ultrastructure, Cd concentration and Cd transporters were measured.
Results  Results showed that the levels of ROS (i.e., superoxide, hydrogen peroxide and lipid peroxidation) were mitigated in +AMF + Cd treatments, especially in +Ri + Cd, compared with −AMF + Cd. For antioxidants, lower levels of catalase, peroxidase and superoxide dismutase, whereas higher levels of glutathione and glutathione peroxidase were observed in +AMF + Cd compared with −AMF + Cd. Chlorophyll content and mesophyll cell ultrastructure were improved by AMF. Cd concentrations in plants were significantly decreased in +AMF + Cd (10 mg Cd kg⁻¹) treatments.
Conclusions  AMF not only decreased Cd accumulation in rice but also regulated the ROS scavenging activities. The variations of ROS and antioxidants were restrained to smaller ranges by AMF. AMF may assist in Cd restriction and thus helping the host to release glutathione for ROS scavenging. Copyright © 2020 Springer Nature Switzerland AG.
Original languageEnglish
JournalPlant and Soil
Early online date17 Aug 2020
DOIs
Publication statusE-pub ahead of print - 17 Aug 2020

Citation

Li, H., Chen, X. W., Wu, L., Luo, N., Huang, W. X., Mo, C. H., . . . Wong, M. H. (2020). Effects of arbuscular mycorrhizal fungi on redox homeostasis of rice under Cd stress. Plant and Soil. Advance online publication. doi: 10.1007/s11104-020-04678-y

Keywords

  • Crop
  • Heavy metal
  • Symbiosis
  • Reactive oxygen species
  • Antioxidant
  • Cd transporter

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