Effects of alpha-lactalbumin or whey protein isolate on muscle damage, muscle pain, and mood states following prolonged strenuous endurance exercise

Lu QIN, Stephen Heung Sang WONG, Fenghua SUN, Yu HUANG, Sinead SHERIDAN, Cindy Hui Ping SIT

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study compared the effect of alpha-lactalbumin and whey protein on muscle damage, muscle pain, and mood states during short term recovery following strenuous prolonged exercise. In a two-stage crossover counterbalanced design, 12 endurance male runners were recruited (age: 30.4 ± 2.8 year, height: 172.7 ± 5.6 cm, body mass: 66.7 ± 6.5 kg, VO2max: 58.0 ± 6.9 ml/kg/min), ran for 90 min at 70% VO2max, and followed by a 4-h recovery. Two treatments (carbohydrate+alpha-lactalbumin, CA; carbohydrate+whey protein isolate, CW) were applied during the main trials. During the first 2-h of recovery, CHO was served at the rate of 0.66 g/kg/h and PRO at 0.34 g/kg/h every 30 min. Creatine kinase (CK), interleukin-6 (IL-6), salivary cortisol, rating of muscle pain, pressure pain threshold (PPT), and mood states were evaluated before (Pre-ex), immediately (Post-ex0), 2 h (Post-ex2h) and 4 h (Post-ex4h) after exercise. 24 h after exercise (Post-ex24h), CK and IL-6, muscle pain, and PPT were evaluated. Compared with Pre-ex, Post-ex24h CK was higher in both trials of CA (398.16 ± 41.37 vs. 184.77 ± 22.68 IU/L, P = 0.039) and CW (418.17 ± 67.86 vs. 202.41 ± 22.26 IU/L, P = 0.037). IL-6 was also higher than Pre-ex at Post-ex0 and Post-ex2h in trials of CA (Post-ex0 vs. Pre-ex0: 7.87 ± 0.74 vs. 1.69 ± 0.23, P < 0.01; Post-ex2h vs. Pre-ex0: 5.39 ± 0.88 vs. 1.69 ± 0.23, P = 0.02) and CW (Post-ex0 vs. Pre-ex0: 8.63 ± 1.06 vs. 1.59 ± 0.19, P < 0.01; Post-ex2h vs. Pre-ex0: 5.75 ± 1.33 vs. 1.59 ± 0.19, P < 0.01). No difference was found in CK and IL-6 between two trials at all time points (all P > 0.05). Compared with Pre-ex0, salivary cortisol was elevated at Post-ex0 in both trials (CA: 0.96 ± 0.13 vs. 0.41 ± 0.05 ng/ml, P < 0.01; CW: 1.15 ± 0.18 vs. 0.43 ± 0.06 ng/ml, P < 0.01) and was lower at Post-ex24h than Pre-ex in CA trial (0.17 ± 0.02 vs. 0.41 ± 0.05 ng/ml, P < 0.01). Compared with CW, PPT was higher at Post-2h in CA trial (31.55 ± 3.09 vs. 26.99 ± 2.32 N/cm2, P < 0.01). Compared with Post-ex0, feeling of fatigue was lower at Post-ex2h (P = 0.014) and Post-ex4h (P < 0.01) in CA, while it was lower at Post-ex4h (P = 0.038) in CW. Compared with CW, feeling of fatigue was marginally lower in the CA trial at Post-ex2h (P = 0.056). In conclusion, compared with the co-ingestion of CHO and whey PRO isolate, co-ingestion of CHO and alpha-lactalbumin reduced sensitivity to the muscle pain, attenuated feeling of fatigue and was more beneficial to reduce the feeling of fatigue and cortisol responses during 4-h recovery following 90-min running at 70% VO2max. Copyright © 2017 Qin, Wong, Sun, Huang, Sheridan and Sit.
Original languageEnglish
Article number754
JournalFrontiers in Physiology
Volume8
DOIs
Publication statusPublished - 2017

Citation

Qin, L., Wong, S. H. S., Sun, F.-H., Huang, Y., Sheridan, S., & Sit, C. H. P. (2017, September). Effects of alpha-lactalbumin or whey protein isolate on muscle damage, muscle pain, and mood states following prolonged strenuous endurance exercise. Frontiers in Physiology, 8, Article 754. Retrieved November 23, 2017, from http://dx.doi.org/10.3389/fphys.2017.00754

Keywords

  • Nutrition
  • Running
  • Muscle recovery
  • Stress
  • Affect

Fingerprint Dive into the research topics of 'Effects of alpha-lactalbumin or whey protein isolate on muscle damage, muscle pain, and mood states following prolonged strenuous endurance exercise'. Together they form a unique fingerprint.