Do arbuscular mycorrhizal fungi affect cadmium uptake kinetics, subcellular distribution and chemical forms in rice?

Hui LI, Na LUO, Li Jun ZHANG, Hai-Ming ZHAO, Yan-Wen LI, Quan-Ying CAI, Ming Hung WONG, Ce-Hui MO

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Abstract

Rice (Oryza sativa L.) plants were inoculated with two species of arbuscular mycorrhizal fungi (AMF) - Rhizophagus intraradices (RI) and Funneliformis mosseae (FM) and grown for 60 days to ensure strong colonization. Subsequently, a short-term hydroponic experiment was carried out to investigate the effects of AMF on cadmium (Cd) uptake kinetics, subcellular distribution and chemical forms in rice exposed to six Cd levels (0, 0.005, 0.01, 0.025, 0.05, 0.1 mM) for three days. The results showed that the uptake kinetics of Cd fitted the Michaelis-Menten model well (R2 > 0.89). AMF significantly decreased the Cd concentrations both in shoots and roots in Cd solutions. Furthermore, the decrement of Cd concentrations by FM was significantly higher than RI treatment in roots. AMF reduced the Cd concentrations markedly in the cell wall fractions at high Cd substrate (≥ 0.025 mM). The main subcellular fraction contributed to Cd detoxification was cell wall at low Cd substrate (< 0.05 mM), while vacuoles at high Cd substrate (≥ 0.05 mM). Moreover, the concentrations and proportions of Cd in inorganic and water-soluble form also reduced by AMF colonization at high Cd substrate (≥ 0.05 mM), both in shoots and roots. This suggested that AMF could convert Cd into inactive forms which were less toxic. Therefore, AMF could enhance rice resistance to Cd through altering subcellular distribution and chemical forms of Cd in rice. Copyright © 2016 Elsevier B.V.
Original languageEnglish
Pages (from-to)1183-1190
JournalScience of the Total Environment
Volume571
Early online dateJul 2016
DOIs
Publication statusPublished - Nov 2016

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Fungi
Cadmium
cadmium
rice
fungus
kinetics
Kinetics
substrate
chemical
distribution
Substrates
colonization
shoot
Cells
Detoxification
Poisons
hydroponics
detoxification

Citation

Li, H., Luo, N., Zhang, L. J., Zhao, H. M., Li, Y. W., Cai, Q. Y., et al. (2016). Do arbuscular mycorrhizal fungi affect cadmium uptake kinetics, subcellular distribution and chemical forms in rice?. Science of The Total Environment, 571, 1183-1190.

Keywords

  • Arbuscular mycorrhizal fungi
  • Heavy metal
  • Rice
  • Mechanism
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