Discovery and functional characterization of novel miRNAs in the marine medaka Oryzias melastigma

Jing-Woei LI, Xiao LIN, Anna TSE, Angela CHEUNG, Ting Fung CHAN, Richard Yuen Chong KONG, Keng Po LAI, Shiu Sun Rudolf WU

Research output: Contribution to journalArticles

5 Citations (Scopus)

Abstract

The marine medaka Oryzias melastigma has often been used as a marine fish model to investigate the biological responses to environmental stresses and pollutants in marine environments. miRNAs are post-transcriptional regulators of many biological processes in a variety of organisms, and have been shown to be affected by environmental stresses, but the novel miRNA profile of marine medaka has not been reported. Using both genome and small RNA sequencings coupled with different bioinformatics analyses, we have discovered 58, 82, 234, and 201 unannotated miRNAs in the brain, liver, ovary and testis tissues of marine medaka, respectively. Furthermore, these novel miRNAs were found to target genes with tissue-specific roles such as neuron development and synaptic transmission in the brain, glucose and fat metabolism in the liver and steroidogenesis in the gonads. We here report, for the first time, novel miRNA profile of marine medaka, which will provide a foundation for future biomarkers and transgenerational studies for the assessment of environmental stresses and pollutions in the marine environments. In a boarder context, our data will provide novel insight into our knowledge of miRNome and miR research. Copyright © 2016 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)106-116
JournalAquatic Toxicology
Volume175
Early online date14 Mar 2016
DOIs
Publication statusPublished - Jun 2016

Citation

Li, J.-W., Lin, X., Tse, A., Cheung, A., Chan., T.F., Kong, R. Y. C., Lai, K. P., & Wu, R. S. S. (2016). Discovery and functional characterization of novel miRNAs in the marine medaka Oryzias melastigma. Aquatic Toxicology, 175, 106-116. doi: 10.1016/j.aquatox.2016.03.013.

Keywords

  • Novel microRNA
  • Marine medaka
  • Genome sequencing
  • Small RNA sequencing
  • Biomarkers

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