Dexamethasone protects RAW264.7 macrophages from growth arrest and apoptosis induced by H₂O₂ through alteration of gene expression patterns and inhibition of nuclear factor-kappa B (NF-κB) activity

Chi-Chun FONG, Yaou ZHANG, Qi ZHANG, Chi-Hung TZANG, Wang-Fun FONG, Shiu Sun Rudolf WU, Mengsu YANG

Research output: Contribution to journalArticles

12 Citations (Scopus)

Abstract

In this study, the effect of dexamethasone, a synthetic glucocorticoid, on H₂O₂ stimulated murine RAW264.7 macrophages was investigated. It was found that dexamethasone protected the cells from apoptosis induced by H₂O₂. A cDNA microarray, which consists of 1000 genes selected from a mouse clone set provided from NIA, was used to study the gene expression profiles involved in the protective effect. Our data show that dexamethasone exerts the anti-apoptosis function by changing the expression patterns of many genes involved inhibiting the up-regulation of apoptosis promoting genes and the down-regulation of cell cycle stimulating genes as well as keeping the up-regulation of cell survival related genes. Our study also revealed that dexamethasone protects RAW264.7 macrophages from H₂O₂ induced apoptosis through blocking nuclear factor-kappa B (NF-κB) activity. Copyright © 2007 Elsevier Ireland Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)16-28
JournalToxicology
Volume236
Issue number1-2
DOIs
Publication statusPublished - 01 Jul 2007

Citation

Fong, C.-C., Zhang, Y., Zhang, Q., Tzang, C.-H., Fong, W.-F., Wu, R. S. S., & Yang, M. (2007). Dexamethasone protects RAW264.7 macrophages from growth arrest and apoptosis induced by H₂O₂ through alteration of gene expression patterns and inhibition of nuclear factor-kappa B (NF-κB) activity. Toxicology, 236(1-2). 16-28. doi: 10.1016/j.tox.2007.03.024

Keywords

  • RAW264.7 macrophages
  • Dexamethasone
  • Hydrogen peroxide
  • Gene expression
  • NF-κB activity

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